Interaction between PII and NifA in Azospirillum brasilense Sp7

被引：8
|
作者：
Chen, SF [1 ]
Du, JP
Wu, LX
Zhao, YS
Li, JL
机构：
[1] China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R China
[2] China Agr Univ, Natl Key Lab Agrobiotechnol, Beijing 100094, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 2003年 / 48卷 / 02期
关键词：
Azospirillum brasilense; yeast two-hybrid system; P-II; NifA;
D O I：
10.1360/03tb9035
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The interaction between P-II and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that P-II directly interacted with the entire NifA protein and its N-terminal domain, but did not interact with the central domain and the C-terminal domain of NifA. No interaction happened if glnB coding for P-II was frame-shift mutated. Pz, a homolog of P-II, had no interation with NifA.
引用
收藏
页码：170 / 174
页数：5
相关论文
共 50 条
  • [1] Interaction between PII and NifA in Azospirillum brasilense Sp7
    CHEN Sanfeng1
    2. National Key Lab. for Agrobiotechnology
    ChineseScienceBulletin, 2003, (02) : 170 - 174
  • [2] Screening of the interacting proteins with NifA in Azospirillum brasilense Sp7 by the yeast two-hybrid system
    CHEN Sanfeng1
    2. Key Laboratory of Agro-Microbial Resource and Application in Agri- culture Ministry
    3. Department of Microbiology
    Chinese Science Bulletin, 2005, (07) : 641 - 646
  • [3] Screening of the interacting proteins with NifA in Azospirillum brasilense Sp7 by the yeast two-hybrid system
    Chen, SF
    Guan, Y
    Tu, R
    Sun, WG
    Li, JL
    CHINESE SCIENCE BULLETIN, 2005, 50 (07): : 641 - 646
  • [4] CONSTRUCTION OF AN AZOSPIRILLUM-BRASILENSE SP7 RECA MUTANT
    CROES, C
    VANDERLEYDEN, J
    MICHIELS, K
    MOLECULAR & GENERAL GENETICS, 1990, 223 (01): : 152 - 155
  • [5] MOLYBDENUM INCORPORATION IN DENITRIFYING AZOSPIRILLUM-BRASILENSE SP7
    CHAURET, C
    BARRAQUIO, WL
    KNOWLES, R
    CANADIAN JOURNAL OF MICROBIOLOGY, 1992, 38 (10) : 1042 - 1047
  • [6] Zinc resistance in Azospirillum brasilense sp7 and its application
    Gowri, PM
    Srivastava, S
    ENVIRONMENTAL BIOTECHNOLOGY: PRINCIPLES AND APPLICATIONS, 1996, : 135 - 143
  • [7] Encapsulation as a response of Azospirillum brasilense sp7 to zinc stress
    Gowri, PM
    Srivastava, S
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1996, 12 (04): : 319 - 322
  • [8] Reduced uptake based zinc resistance in Azospirillum brasilense sp7
    Gowri, PM
    Srivastava, S
    CURRENT SCIENCE, 1996, 71 (02): : 139 - 142
  • [9] Cloning and characterization of a fur homologue from Azospirillum brasilense Sp7
    Alahari, A
    Tripathi, AK
    Le Rudulier, D
    CURRENT MICROBIOLOGY, 2006, 52 (02) : 123 - 127
  • [10] Reduced uptake based zinc resistance in Azospirillum brasilense sp7
    Curr Sci, 2 (139):
12345