Interaction between PII and NifA in Azospirillum brasilense Sp7

被引：8

作者：

Chen, SF ^{[1
]}

Du, JP

Wu, LX

Zhao, YS

Li, JL

机构：

[1] China Agr Univ, Dept Microbiol, Beijing 100094, Peoples R China

[2] China Agr Univ, Natl Key Lab Agrobiotechnol, Beijing 100094, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2003年 / 48卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Azospirillum brasilense; yeast two-hybrid system; P-II; NifA;

D O I：

10.1360/03tb9035

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The interaction between P-II and NifA in A. brasilense Sp7 was investigated by using the yeast two-hybrid system. Our experimental results showed that P-II directly interacted with the entire NifA protein and its N-terminal domain, but did not interact with the central domain and the C-terminal domain of NifA. No interaction happened if glnB coding for P-II was frame-shift mutated. Pz, a homolog of P-II, had no interation with NifA.

引用

页码：170 / 174

页数：5

共 12 条

[1] PII signal transduction proteins, pivotal players in microbial nitrogen control [J].

Arcondéguy, T ;

Jack, R ;

Merrick, M .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2001, 65 (01) :80-+

[2] NUCLEOTIDE-SEQUENCE OF A 24,206-BASE-PAIR DNA FRAGMENT CARRYING THE ENTIRE NITROGEN-FIXATION GENE-CLUSTER OF KLEBSIELLA-PNEUMONIAE [J].

ARNOLD, W ;

RUMP, A ;

KLIPP, W ;

PRIEFER, UB ;

PUHLER, A .

JOURNAL OF MOLECULAR BIOLOGY, 1988, 203 (03) :715-738

[3] Control of Azospirillum brasilense NifA activity by PII:: effect of replacing Tyr residues of the NifA N-terminal domain on NifA activity [J].

Arsène, F ;

Kaminski, PA ;

Elmerich, C .

FEMS MICROBIOLOGY LETTERS, 1999, 179 (02) :339-343

[4] Modulation of NifA activity by P-II in Azospirillum brasilense: Evidence for a regulatory role of the NifA N-terminal domain [J].

Arsene, F ;

Kaminski, PA ;

Elmerich, C .

JOURNAL OF BACTERIOLOGY, 1996, 178 (16) :4830-4838

[5]

*CLONTECH LAB, 1996, CLONT MATCHM LEXA 2

[6] Structural homologues PII and Pz of Azospirillum brasilense provide intracellular signalling for selective regulation of various nitrogen-dependent functions [J].

de Zamaroczy, M .

MOLECULAR MICROBIOLOGY, 1998, 29 (02) :449-463

[7]

FANCELLI S, 1994, FEMS MICROBIOL LETT, V115, P57

[8]

James P, 1996, GENETICS, V144, P1425

[9] NITROGEN CONTROL IN BACTERIA [J].

MERRICK, MJ ;

EDWARDS, RA .

MICROBIOLOGICAL REVIEWS, 1995, 59 (04) :604-&

[10] NITROGEN CONTROL OF THE NIF REGULON IN KLEBSIELLA-PNEUMONIAE - INVOLVEMENT OF THE NTRA GENE AND ANALOGIES BETWEEN NTRC AND NIFA [J].

MERRICK, MJ .

EMBO JOURNAL, 1983, 2 (01) :39-44

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