Mycobacterium senriense sp. nov., a slowly growing, non-scotochromogenic species, isolated from sputum of an elderly man

被引：3

作者：

Abe, Yuko ^{[1
]}

Fukushima, Kiyoharu ^{[1
,2
,3
]}

Matsumoto, Yuki ^{[4
]}

Niitsu, Takayuki ^{[1
]}

Nabeshima, Hiroshi ^{[3
,5
]}

Nagahama, Yasuharu ^{[3
,5
]}

Akiba, Eri ^{[2
]}

Haduki, Kako ^{[2
]}

Saito, Haruko ^{[2
]}

Nitta, Tadayoshi ^{[2
]}

Kawano, Akira ^{[2
]}

Tanaka, Michio ^{[6
]}

Matsuki, Takanori ^{[2
]}

Motooka, Daisuke ^{[4
]}

Tsujino, Kazuyuki ^{[2
]}

Miki, Keisuke ^{[2
]}

Nakamura, Shota ^{[4
]}

Iida, Tetsuya ^{[6
]}

Kida, Hiroshi ^{[2
]}

机构：

[1] Osaka Univ, Dept Resp Med & Clin Immunol, Grad Sch Med, 2-2 Yamadaoka, Suita, Osaka, Japan

[2] Natl Hosp Org, Dept Resp Med, Osaka Toneyama Med Ctr, 5-1-1 Toneyama, Toyonaka, Osaka, Japan

[3] Osaka Univ, World Premier Inst Immunol Frontier Res Ctr WPI I, Lab Host Def, 3-1 Yamadaoka, Suita, Osaka, Japan

[4] Osaka Univ, Genome Informat Res Ctr, Res Inst Microbial Dis RIMD, Dept Infect Metagen, 3-1 Yamadaoka, Osaka, Japan

[5] Otsuka Pharmaceut Co Ltd, Fujii Mem Res Inst, Otsu, Shiga 5200106, Japan

[6] Osaka Univ, Res Inst Microbial Dis RIMD, Pathogen Microbes Repository Unit, 3-1 Yamadaoka, Suita, Osaka, Japan

来源：

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY | 2022年 / 72卷 / 05期

关键词：

Mycobacterium avium complex; mycobacteria; novel;

D O I：

10.1099/ijsem.0.005378

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A slowly growing mycobacteria, identified as strain TY59(T), was isolated from sputum of an elderly man with pneumonia. Sequencing of the 16S rRNA gene indicated that this strain was similar to members of the Mycobacterium avium complex and closely related species. Strain TY59(T) has highest 16S rRNA gene sequence similarities to the type strains of Mycobacterium colombiense (99.80% sequence similarity), Mycobacterium vulneris (99.74 %), Mycobacterium timonense (99.54%), Mycobacterium avium subsp. avium (99.54%) and Mycobacterium avium subsp. silvaticum (99.54%). Analysis of the internal transcribed spacer (ITS) and DNA-directed RNA polymerase subunit beta (rpoB) sequences gave similar results to the 16S rRNA gene analysis. The closest species to strain TY59(T) were M. colombiense and M. vulneris with 97.90-98.25 % identity in ITS and 96.4-96.6% in rpoB. The strain's 65 kDa heat shock protein (hsp65) gene was different from those of M. vulneris, M. colombiense and M. avium subsp. silvaticum with 72.4-74.2 % identity. Average nucleotide identity results showed a 93.4 % match to M. vulneris as the maximum value. Phenotypically, the non-chromogenicity, rough colonies, growth at 42 degrees C, negative results for nitrate reduction, beta-glucosidase and Tween 80 hydrolysis, and positive results for catalase activity set this strain apart from closely related species. We propose that Mycobacterium senriense sp. nov. is a novel species of slowly growing mycobacteria. The type strain is TY59(T) (RIMD 1371001(T)=CIP 111917(T)).

引用

页数：5

共 15 条

[1]

Abe Y., 2020, INT J MOL SCI, V21, P12

[2] Draft Genome Sequence of Mycobacterium vulneris DSM 45247(T) [J].