Effect of nutrient media on axillary shoot proliferation and preconditioning for adventitious shoot regeneration of pears

被引：47
作者：
Bell, Richard L. [1 ]
Srinivasan, Chinnathambi [1 ]
Lomberk, Delores [2 ]
机构：
[1] ARS, USDA, Appalachian Fruit Res Stn, Kearneysville, WV 25430 USA
[2] ARS, USDA, Lake Buena Vista, FL 32830 USA
来源：
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT | 2009年 / 45卷 / 06期
关键词：
'Bartlett'; 'Beurre Bosc'; Biotechnology; Plant tissue culture; Pyrus communis; INVITRO-PROPAGATION; MICROPROPAGATION;
D O I：
10.1007/s11627-009-9196-8
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The influence of the nutrient composition of plant tissue culture media on axillary shoot proliferation and their preconditioning effect on subsequent adventitious shoot regeneration from pear leaves was investigated. The goal was to improve both micropropagation and regeneration of 'Bartlett' and 'Beurre Bosc' pear cultivars. Driver-Kuniyuki walnut (DKW) and Quoirin and Lepoivre (QL) nutrient media were found to be superior to Murashige and Skoog (MS) and Woody Plant Medium (WPM) for axillary shoot proliferation. Shoots on WPM exhibited some chlorosis. Axillary shoot culture on DKW would be preferred to that on QL due to the production of excessively short thin shoots on the latter medium. DKW also was superior to QL and MS for production of young expanding leaves for use as explants in adventitious regeneration. Leaf explants derived from shoot proliferation cultures grown on DKW or QL media produced more adventitious shoots than leaf explants from MS.
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页码：708 / 714
页数：7
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