Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks

被引:30
作者
Xie, Mingjun [1 ,2 ]
Yu, Kang [1 ,2 ]
Sun, Yuan [1 ,2 ]
Shao, Lei [1 ,2 ]
Nie, Jing [1 ,2 ]
Gao, Qing [1 ,2 ]
Qiu, Jingjiang [3 ]
Fu, Jianzhong [1 ,2 ]
Chen, Zichen [1 ,2 ]
He, Yong [1 ,2 ]
机构
[1] Zhejiang Univ, Sch Mech Engn, State Key Lab Fluid Power & Mechatron Syst, Hangzhou, Zhejiang, Peoples R China
[2] Zhejiang Univ, Sch Mech Engn, Key Lab 3D Printing Proc & Equipment Zhejiang Pro, Hangzhou, Zhejiang, Peoples R China
[3] Zhengzhou Univ, Sch Mech & Safety Engn, Hangzhou, Zhejiang, Peoples R China
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2019年 / 154期
基金
中国国家自然科学基金;
关键词
Bioengineering; Issue; 154; 3D bioprinting; gelatin methacryloyl; GelMA; microsphere; microfiber; digital light processing; DLP; microfluidic chip; MICROFLUIDIC CHIP; MICROSPHERES; FABRICATION;
D O I
10.3791/60545
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Gelatin methacryloyl (GelMA) has become a popular biomaterial in the field of bioprinting. The derivation of this material is gelatin, which is hydrolyzed from mammal collagen. Thus, the arginine-glycine-aspartic acid (RGD) sequences and target motifs of matrix metalloproteinase (MMP) remain on the molecular chains, which help achieve cell attachment and degradation. Furthermore, formation properties of GelMA are versatile. The methacrylamide groups allow a material to become rapidly crosslinked under light irradiation in the presence of a photoinitiator. Therefore, it makes great sense to establish suitable methods for synthesizing three-dimensional (3D) structures with this promising material. However, its low viscosity restricts GelMA's printability. Presented here are methods to carry out 3D bioprinting of GelMA hydrogels, namely the fabrication of GelMA microspheres, GelMA fibers, GelMA complex structures, and GelMA-based microfluidic chips. The resulting structures and biocompatibility of the materials as well as the printing methods are discussed. It is believed that this protocol may serve as a bridge between previously applied biomaterials and GelMA as well as contribute to the establishment of GelMA-based 3D architectures for biomedical applications.
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页数:6
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