Gene targeting in livestock: a preview

被引：33
作者：
Clark, AJ [1 ]
Burl, S [1 ]
Denning, C [1 ]
Dickinson, P [1 ]
机构：
[1] Roslin Inst, Roslin EH25 9PS, Midlothian, Scotland
来源：
TRANSGENIC RESEARCH | 2000年 / 9卷 / 4-5期
关键词：
biotechnology; gene targeting; livestock; nuclear transfer; somatic cells;
D O I：
10.1023/A:1008974616402
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Until recently genetically modified livestock could only be generated by pronuclear injection. The discovery that animals can be cloned by nuclear transfer from cultured somatic cells means that it will now be possible to achieve gene targeting in these species. We discuss current developments in NT, the prospects and technical challenges for introducing targeted changes into the germline by this route, and the types of application for which this new technology will be used.
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收藏
页码：263 / 275
页数：13
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