A time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) activity

被引：18

作者：

Cui, Wei ^{[1
]}

Parker, Laurie L. ^{[1
]}

机构：

[1] Purdue Univ, Coll Pharm, Purdue Ctr Canc Res, Dept Med Chem & Mol Pharmacol, W Lafayette, IN 47907 USA

来源：

CHEMICAL COMMUNICATIONS | 2015年 / 51卷 / 02期

基金：

美国国家卫生研究院;

关键词：

C-MET; PHOSPHORYLATION; CANCER; SENSITIZATION; INHIBITORS; BINDING; DOMAIN;

D O I：

10.1039/c4cc07453j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel time-resolved luminescence biosensor assay for anaplastic lymphoma kinase (ALK) was developed. We used a straightforward strategy to modify a known ALK substrate into a peptide biosensor that can accommodate terbium luminescence sensitization upon its phosphorylation by ALK. Since this strategy is generalizable, this high-throughput screening compatible assay serves as an example for development of other kinase assays that employ terbium luminescence as a read-out.

引用

收藏

页码：362 / 365

页数：4

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