Secondary Ion Mass Spectrometry Imaging Reveals Changes in the Lipid Structure of the Plasma Membranes of Hippocampal Neurons following Drugs Affecting Neuronal Activity

被引：9

作者：

Aguei-Gonzalez, Paola ^{[1
,2
]}

Bao Guobin ^{[3
]}

de Castro, Maria A. Gomes ^{[1
]}

Rizzoli, Silvio O. ^{[1
,2
]}

Phan, Nhu T. N. ^{[1
,2
,4
]}

机构：

[1] Univ Med Ctr Gottingen, Dept Neuro & Sensory Physiol, D-37073 Gottingen, Germany

[2] Univ Med Ctr Gottingen, Ctr Biostruct Imaging Neurodegenerat, D-37075 Gottingen, Germany

[3] Univ Med Ctr Gottingen, Dept Pharmacol & Toxicol, D-37075 Gottingen, Germany

[4] Univ Gothenburg, Dept Chem & Mol Biol, S-41296 Gothenburg, Sweden

来源：

ACS CHEMICAL NEUROSCIENCE | 2021年 / 12卷 / 09期

基金：

瑞典研究理事会; 欧洲研究理事会;

关键词：

ToF-SIMS; mass spectrometry imaging; lipids; neurons; membranes; CERAMIDE; PHOSPHATIDYLSERINE; BRAIN; 2ND-MESSENGER; AFFINITY; RECEPTOR; RAFTS; CYCLE; ACID;

D O I：

10.1021/acschemneuro.1c00031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cellular functions of lipids in the neuronal plasma membranes have been increasingly acknowledged, particularly their association to neuronal processes and synaptic plasticity. However, the knowledge of their regulatory mechanisms in neuronal cells remains sparse. To address this, we investigated the lipid organization of the plasma membranes of hippocampal neurons in relation to neuronal activity using secondary ion mass spectrometry imaging. The neurons were treated with drugs, particularly tetrodotoxin (TTX) and bicuculline (BIC), to induce chronic activation and silencing. Distinct lipid organization was found in the plasma membrane of the cell body and the neurites. Moreover, significant alterations of the levels of the membrane lipids, especially ceramides, phosphatidylserines, phosphatidic acids, and triacylglycerols, were observed under the TTX and BIC treatments. We suggest that many types of membrane lipids are affected by, and may be involved in, the regulation of neuronal function.

引用

页码：1542 / 1551

页数：10