Clot Lysis Capacity of Andrographis paniculata and its Pure Compound Andrographolide

Background: Andrographis paniculata and its main components andrographolide (AGL) have been reported for their promising cardio-protective effects in experimental animals. Aim: To investigate clot lysis activity of A. paniculata and AGL. Materials and Methods: The clot lysis activity of the aqueous A. paniculata stem extract (AAP) and AGL was investigated on clotted human blood by using streptokinase (SKE) as a standard drug. Results: Both AAP and AGL showed a concentration-dependent clot lysis activity. AAP showed the highest clot lysis activity at 100 mu L (eq. 10% w/v) (54.12 +/- 1.22%), while AGL showed at 100 mu L/mL (58.62 +/- 2.02%). The half maximal effective concentration (EC50) values of AAP and AGL were 87.32 +/- 0.37 mu L (eq. 8.732% w/v) and 79.74 +/- 0.31 mu L/mL, respectively. The netagive controls showed negligible clot lysis capacities, while the standard SKE showed 81.54 +/- 0.78% clot lysis activity. Conclusion: AAP and its pure component NGL exhibited significant clot lysis activity on human clotted blood and may be good candidates for the treatment of atherothrombosis.