A Fluorogenic Probe for Ultrafast and Reversible Detection of Formaldehyde in Neurovascular Tissues

被引：46

作者：

Liang, Xing-Guang ^{[1
,3
]}

Chen, Bo ^{[2
]}

Shao, Ling-Xiao ^{[1
]}

Cheng, Juan ^{[1
]}

Huang, Ming-Zhu ^{[3
]}

Chen, Yu ^{[3
]}

Hu, Yong-Zhou ^{[1
]}

Han, Yi-Feng ^{[2
]}

Han, Feng ^{[1
]}

Li, Xin ^{[1
]}

机构：

[1] Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China

[2] Zhejiang Sci Tech Univ, Dept Chem, Hangzhou 310018, Zhejiang, Peoples R China

[3] Zhejiang Univ, Affiliated Hosp 1, Sch Med, Cent Lab, Hangzhou 310003, Zhejiang, Peoples R China

来源：

THERANOSTICS | 2017年 / 7卷 / 08期

关键词：

fluorescent probe; formaldehyde; neurodegenerative disorder; neurovascular cell; SENSITIVE AMINE OXIDASE; 2-PHOTON FLUORESCENT-PROBE; ENDOGENOUS FORMALDEHYDE; LIVING CELLS; BRAIN; URINE; BLOOD; RAT; QUANTIFICATION; CHROMATOGRAPHY;

D O I：

10.7150/thno.19554

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Formaldehyde (FA) is endogenously produced in live systems and has been implicated in a diverse array of pathophysiological processes. To disentangle the detailed molecular mechanisms of FA biology, a reliable method for monitoring FA changes in live cells would be indispensable. Although there have been several fluorescent probes reported to detect FA, most are limited by the slow detection kinetics and the intrinsic disadvantage of detecting FA in an irreversible manner which may disturb endogenous FA homeostasis. Herein we developed a coumarin-hydrazonate based fluorogenic probe (PFM) based on a finely-tailored stereoelectronic effect. PFM could respond to FA swiftly and reversibly. This, together with its desirable specificity and sensitivity, endows us to track endogenous FA in live neurovascular cells with excellent temporal and spatial resolution. Further study in the brain tissue imaging showed the first direct observation of aberrant FA accumulation in cortex and hippocampus of Alzheimer's mouse model, indicating the potential of PFM as a diagnostic tool.

引用

页码：2305 / 2313

页数：9

共 43 条

[1] Pathogen Cell-to-Cell Variability Drives Heterogeneity in Host Immune Responses [J].

Avraham, Roi ;

Haseley, Nathan ;

Brown, Douglas ;

Penaranda, Cristina ;

Jijon, Humberto B. ;

Trombetta, John J. ;

Satija, Rahul ;

Shalek, Alex K. ;

Xavier, Ramnik J. ;

Regev, Aviv ;

Hung, Deborah T. .

CELL, 2015, 162 (06) :1309-1321

[2] An Aza-Cope Reactivity-Based Fluorescent Probe for Imaging Formaldehyde in Living Cells [J].

Brewer, Thomas F. ;

Chang, Christopher J. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (34) :10886-10889

[3] Spatially resolved, highly multiplexed RNA profiling in single cells [J].

Chen, Kok Hao ;

Boettiger, Alistair N. ;

Moffitt, Jeffrey R. ;

Wang, Siyuan ;

Zhuang, Xiaowei .

SCIENCE, 2015, 348 (6233)

[4]

Dean KM, 2014, NAT CHEM BIOL, V10, P512, DOI [10.1038/nchembio.1556, 10.1038/NCHEMBIO.1556]

[5]

Demchenko AlexanderP., 2009, INTRO FLUORESCENCE S

[6] REGIONAL AND SUBCELLULAR-DISTRIBUTION OF PROTEIN CARBOXYMETHYLASE IN BRAIN AND OTHER TISSUES [J].

DILIBERTO, EJ ;

AXELROD, J .

JOURNAL OF NEUROCHEMISTRY, 1976, 26 (06) :1159-1165

[7] Quantitative analysis by gas chromatography of volatile carbonyl compounds in expired air from mice and human [J].

Ebeler, SE ;

Clifford, AJ ;

Shibamoto, T .

JOURNAL OF CHROMATOGRAPHY B, 1997, 702 (1-2) :211-215

[8] Overexpression of semicarbazide sensitive amine oxidase in the cerebral blood vessels in patients with Alzheimer's disease and cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy [J].

Ferrer, I ;

Lizcano, JM ;

Hernández, M ;

Unzeta, M .

NEUROSCIENCE LETTERS, 2002, 321 (1-2) :21-24

[9] An ultra-fast illuminating fluorescent probe for monitoring formaldehyde in living cells, shiitake mushrooms, and indoors [J].

He, Longwei ;

Yang, Xueling ;

Ren, Mingguang ;

Kong, Xiuqi ;

Liu, Yong ;

Lin, Weiying .

CHEMICAL COMMUNICATIONS, 2016, 52 (61) :9582-9585

[10] A ratiometric fluorescent formaldehyde probe for bioimaging applications [J].

He, Longwei ;

Yang, Xueling ;

Liu, Yong ;

Kong, Xiuqi ;

Lin, Weiying .

CHEMICAL COMMUNICATIONS, 2016, 52 (21) :4029-4032

← 1 2 3 4 5 →