Self-resistance mechanism in Streptomyces peucetius: Overexpression of drrA, drrB and drrC for doxorubicin enhancement

被引：63

作者：

Malla, Sailesh ^{[1
]}

Niraula, Narayan Prasad ^{[1
]}

Liou, Kwangkyoung ^{[1
]}

Sohng, Jae Kyung ^{[1
]}

机构：

[1] SunMoon Univ, Dept Pharmaceut Engn, IBR, Asan 336708, Chungnam, South Korea

来源：

MICROBIOLOGICAL RESEARCH | 2010年 / 165卷 / 04期

关键词：

Doxorubicin; Overproduction; Resistance gene; Self-resistance mechanism; Streptomyces peucetius; ABC TRANSPORTER; GENE-CLUSTER; DAUNORUBICIN; EXPRESSION; SECRETION; PROTEINS; SYNTHASE;

D O I：

10.1016/j.micres.2009.04.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The resistance genes drrABC from Streptomyces peucetius ATCC 27952 were cloned into the plBR25 expression vector under a strong ermE* promoter to enhance doxorubicin (DXR) production. The recombinant expression plasmids, pDrrAB25, pDrrC25 and pDrrABC25, were constructed to overexpress drrAB, drrC and drrABC, respectively, in S. peucetius ATCC 27952. The recombinant strains produced more DXR than the parental strain: a 2.2-fold increase with pDrrAB25, a 5.1-fold increase with pDrrC25, and a 2.4-fold increase with pDrrABC25. We also studied the relative ratios of doxorubicin, daunorubicin and epsilon-rhodonnycinone produced in these recombinant strains. (c) 2009 Elsevier GmbH. All rights reserved.

引用

页码：259 / 267

页数：9

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