Design of self-processing antimicrobial peptides for plant protection

被引：155
作者：
Powell, WA [1 ]
Catranis, CM [1 ]
Maynard, CA [1 ]
机构：
[1] SUNY Syracuse, Coll Environm Sci & Forestry, Syracuse, NY 13210 USA
来源：
LETTERS IN APPLIED MICROBIOLOGY | 2000年 / 31卷 / 02期
关键词：
D O I：
10.1046/j.1365-2672.2000.00782.x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Small antimicrobial peptides are excellent candidates for inclusion in self-processing proteins that could be used to confer pathogen resistance in transgenic plants. Antimicrobial peptides as small as 22 amino acids in length have been designed to incorporate the residual amino acids left from protein processing by the tobacco etch virus'(TEVs') NIa protease. Also, by minimizing the length of these peptides and the number of highly hydrophobic residues, haemolytic activity was reduced without affecting the peptide's antimicrobial activity.
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页码：163 / 168
页数：6
相关论文
共 17 条
  • [1] AN IMPROVED DEFINED MEDIUM FOR GROWTH OF ENDOTHIA-PARASITICA
    ANAGNOSTAKIS, SL
    [J]. MYCOLOGIA, 1982, 74 (05) : 826 - 830
  • [2] STRUCTURE-FUNCTION STUDIES OF AMPHIPHILIC ANTIBACTERIAL PEPTIDES
    BESSALLE, R
    GOREA, A
    SHALIT, I
    METZGER, JW
    DASS, C
    DESIDERIO, DM
    FRIDKIN, M
    [J]. JOURNAL OF MEDICINAL CHEMISTRY, 1993, 36 (09) : 1203 - 1209
  • [3] A VIRAL CLEAVAGE SITE CASSETTE - IDENTIFICATION OF AMINO-ACID SEQUENCES REQUIRED FOR TOBACCO ETCH VIRUS POLYPROTEIN PROCESSING
    CARRINGTON, JC
    DOUGHERTY, WG
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (10) : 3391 - 3395
  • [4] Simultaneous accumulation of multiple viral coat proteins from a TEV-NIa based expression vector
    Ceriani, MF
    Marcos, JF
    Hopp, HE
    Beachy, RN
    [J]. PLANT MOLECULAR BIOLOGY, 1998, 36 (02) : 239 - 248
  • [5] SYNTHETIC MAGAININ ANALOGS WITH IMPROVED ANTIMICROBIAL ACTIVITY
    CHEN, HC
    BROWN, JH
    MORELL, JL
    HUANG, CM
    [J]. FEBS LETTERS, 1988, 236 (02) : 462 - 466
  • [6] Hydrophobicity, hydrophobic moment and angle subtended by charged residues modulate antibacterial and haemolytic activity of amphipathic helical peptides
    Dathe, M
    Wieprecht, T
    Nikolenko, H
    Handel, L
    Maloy, WL
    MacDonald, DL
    Beyermann, M
    Bienert, M
    [J]. FEBS LETTERS, 1997, 403 (02): : 208 - 212
  • [7] BIOCHEMICAL AND MUTATIONAL ANALYSIS OF A PLANT-VIRUS POLYPROTEIN CLEAVAGE SITE
    DOUGHERTY, WG
    CARRINGTON, JC
    CARY, SM
    PARKS, TD
    [J]. EMBO JOURNAL, 1988, 7 (05) : 1281 - 1287
  • [8] THE HYDROPHOBIC MOMENT DETECTS PERIODICITY IN PROTEIN HYDROPHOBICITY
    EISENBERG, D
    WEISS, RM
    TERWILLIGER, TC
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (01): : 140 - 144
  • [9] STRUCTURE-ACTIVITY STUDIES ON MAGAININS AND OTHER HOST-DEFENSE PEPTIDES
    MALOY, WL
    KARI, UP
    [J]. BIOPOLYMERS, 1995, 37 (02) : 105 - 122
  • [10] IN-VITRO CHARACTERIZATION OF A CASSETTE TO ACCUMULATE MULTIPLE PROTEINS THROUGH SYNTHESIS OF A SELF-PROCESSING POLYPEPTIDE
    MARCOS, JF
    BEACHY, RN
    [J]. PLANT MOLECULAR BIOLOGY, 1994, 24 (03) : 495 - 503
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