Construction of fluorescent biosensing system based on DNA templated quantum dots- graphene oxide interactions for detecting carcinoembryonic antigen

被引：11

作者：

Zheng, Jiyao ^{[1
]}

Di, Ya ^{[1
]}

Gao, Liming ^{[1
]}

Kong, Xianglei ^{[1
]}

Zheng, Yue ^{[1
]}

Han, Junling ^{[1
]}

Wang, Jidong ^{[2
]}

机构：

[1] Hebei Med Univ, Hosp Qinhuangdao 1, Qinhuangdao, Hebei, Peoples R China

[2] Yanshan Univ, Sch Environm & Chem Engn, Hebei Key Lab Appl Chem, Qinhuangdao, Hebei, Peoples R China

来源：

MATERIALS TECHNOLOGY | 2022年 / 37卷 / 12期

基金：

中国国家自然科学基金;

关键词：

Label-free aptamer; 'signal-on' fluorescent biosensor; CEA detection; graphene oxide; quantum dots; IMMUNOSENSOR; CEA; NANOPARTICLES; SEMICONDUCTOR; SIGNAL;

D O I：

10.1080/10667857.2021.1943120

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Carcinoembryonic antigen (CEA) is usually utilised as a reliable diagnostic and predictive marker of gastric cancer (GC). Herein, a target-responsive, label-free aptamer 'signal-on' fluorescent biosensing system was developed based on the synthesis of DNA-templated quantum dots via one-pot route and interactions with graphene oxide. CEA triggered aptamer-DNA hairpins hybridisation to release DNA to hybridise with DNA-CdTe QDs on GO and led to the fluorescence recovery of system with a 'signal-on' status. The fluorescence intensity was proportionate to CEA concentration, and highly sensitive sensing platform for detecting CEA was achieved with a wide linear range (5-250 ng/mL) and a low detection limit (1.17 ng/mL). This sensing strategy, the targeted detection of aptamer for CEA, provided a new direction in the detection of protein and construction of fluorescence biosensors.

引用

页码：2116 / 2122

页数：7

共 30 条

[1] Perspectives on the physical chemistry of semiconductor nanocrystals [J].

Alivisatos, AP .

JOURNAL OF PHYSICAL CHEMISTRY, 1996, 100 (31) :13226-13239

[2] Highly Sensitive DNA Sensor Based on Upconversion Nanoparticles and Graphene Oxide [J].

Alonso-Cristobal, P. ;

Vilela, P. ;

El-Sagheer, A. ;

Lopez-Cabarcos, E. ;

Brown, T. ;

Muskens, O. L. ;

Rubio-Retama, J. ;

Kanaras, A. G. .

ACS APPLIED MATERIALS & INTERFACES, 2015, 7 (23) :12422-12429

[3] Global Burden of 5 Major Types of Gastrointestinal Cancer [J].

Arnold, Melina ;

Abnet, Christian C. ;

Neale, Rachel E. ;

Vignat, Jerome ;

Giovannucci, Edward L. ;

McGlynn, Katherine A. ;

Bray, Freddie .

GASTROENTEROLOGY, 2020, 159 (01) :335-+

[4] Redox-Switchable Binding Properties of the ATP-Aptamer [J].

Biniuri, Yonatan ;

Luo, Guo-Feng ;

Fadeev, Michael ;

Wulf, Verena ;

Willner, Itamar .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2019, 141 (39) :15567-15576

[5] Over-Reduction-Controlled Mixed-Valent Manganese Oxide with Tunable Mn2+/Mn3+ Ratio for High-Performance Asymmetric Supercapacitor with Enhanced Cycling Stability [J].

Fang, Zixun ;

Xu, Ming ;

Li, Qing ;

Qi, Man ;

Xu, Tongtong ;

Niu, Zhimin ;

Qu, Nianrui ;

Gu, Jianmin ;

Wang, Jidong ;

Wang, Desong .

LANGMUIR, 2021, 37 (08) :2816-2825

[6] EFFECTS OF AQUEOUS NEUTRAL-SALT SOLUTIONS ON MELTING TEMPERATURES OF DEOXYRIBONUCLEIC ACIDS [J].

GRUENWEDEL, DW ;

HSU, CH ;

LU, DS .

BIOPOLYMERS, 1971, 10 (01) :47-+

[7] Determination of Cisplatin Cross-Linked Hyaluronic Acid (CPHA) Hydrogel and DNA Using the Fluorescent Response from Mercaptopropionic Acid (MPA) Capped Cadmium Telluride Quantum Dots (CdTe QDs) [J].

Jin, Penghui ;

Ma, Daoqing ;

Gao, Yabiao ;

Wang, Libin ;

Gao, Zhihong ;

Zhang, Yating ;

Liu, Mingxin ;

Xu, Jingying ;

Wang, Jidong .

ANALYTICAL LETTERS, 2021, 54 (15) :2411-2422

[8] An ultrasensitive fluorescence aptasensor for carcino-embryonic antigen detection based on fluorescence resonance energy transfer from upconversion phosphors to Au nanoparticles [J].

Li, Xiang-Hui ;

Sun, Wei-Ming ;

Wu, Juan ;

Gao, Yao ;

Chen, Jing-Hua ;

Chen, Min ;

Ou, Qi-Shui .

ANALYTICAL METHODS, 2018, 10 (13) :1552-1559

[9] Graphene Oxide as a Bifunctional Material toward Superior RNA Protection and Extraction [J].

Liao, Yuhui ;

Zhou, Xiaoming ;

Fu, Yu ;

Xing, Da .

ACS APPLIED MATERIALS & INTERFACES, 2018, 10 (36) :30227-30234

[10] Heating Drives DNA to Hydrophobic Regions While Freezing Drives DNA to Hydrophilic Regions of Graphene Oxide for Highly Robust Biosensors [J].

Liu, Biwu ;

Zhao, Yu ;

Jia, Yu ;

Liu, Juewen .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2020, 142 (34) :14702-14709

← 1 2 3 →