P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity

被引：29

作者：

Akhter, Mohammad S. ^{[1
]}

Uddin, Mohammad A. ^{[1
]}

Kubra, Khadeja-Tul ^{[1
]}

Barabutis, Nektarios ^{[1
]}

机构：

[1] Univ Louisiana Monroe, Coll Pharm, Sch Basic Pharmaceut & Toxicol Sci, 1800 Bienville Dr, Monroe, LA 71201 USA

来源：

JOURNAL OF PHARMACOLOGICAL SCIENCES | 2019年 / 141卷 / 01期

关键词：

Cerebral stroke; Inflammation; Barrier function; OXIDATIVE STRESS; BARRIER FUNCTION; P53;

D O I：

10.1016/j.jphs.2019.09.008

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Dysregulation of the blood brain barrier due to oxidative stress causes neurological disorders, such as Alzheimer's and Parkinson's disease. We employed brain microvascular endothelial cells; to investigate the effects of P53 towards the lipid oxidation of the BBB. P53 reduction by LPS, siRNA for P53 and Pifithrin increased the expression levels of malondialdehyde, a marker of oxidative stress and lipid peroxidation. Furthermore, P53 suppression impaired the permeability of the BBB monolayers. In contrast, P53 induction by Nutlin and Hsp90 inhibitor AUY922 enhanced the BBB function. In conclusion, P53 supports BBB integrity, at least in part, by reducing lipid peroxidation. (C) 2019 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.

引用

页码：83 / 85

页数：3

共 15 条

[1] Unfolded protein response regulates P53 expression in the pulmonary endothelium [J].

Akhter, Mohammad S. ;

Uddin, Mohammad A. ;

Barabutis, Nektarios .

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 2019, 33 (10)

[2] Wild-type p53 enhances endothelial barrier function by mediating RAC1 signalling and RhoA inhibition [J].

Barabutis, Nektarios ;

Dimitropoulou, Christiana ;

Gregory, Betsy ;

Catravas, John D. .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2018, 22 (03) :1792-1804

[3] Antioxidant activity of growth hormone-releasing hormone antagonists in LNCaP human prostate cancer line [J].

Barabutis, Nektarios ;

Schally, Andrew V. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (51) :20470-20475

[4]

Choi Sunga, 2016, Chonnam Med J, V52, P75, DOI 10.4068/cmj.2016.52.2.75

[5] The Inflammatory Response After Ischemic Stroke: Targeting β2 and β1 Integrins [J].

Edwards, Danielle N. ;

Bix, Gregory J. .

FRONTIERS IN NEUROSCIENCE, 2019, 13

[6] Biological markers of oxidative stress: Applications to cardiovascular research and practice [J].

Ho, Edwin ;

Galougahi, Keyvan Karimi ;

Liu, Chia-Chi ;

Bhindi, Ravi ;

Figtree, Gemma A. .

REDOX BIOLOGY, 2013, 1 (01) :483-491

[7] p53-induced up-regulation of MnSOD and GPx but not catalase increases oxidative stress and apoptosis [J].

Hussain, SP ;

Amstad, P ;

He, PJ ;

Robles, A ;

Lupold, S ;

Kaneko, I ;

Ichimiya, M ;

Sengupta, S ;

Mechanic, L ;

Okamura, S ;

Hofseth, LJ ;

Moake, M ;

Nagashima, M ;

Forrester, KS ;

Harris, CC .

CANCER RESEARCH, 2004, 64 (07) :2350-2356

[8] Involvement of MnSOD Alal6Val polymorphism in epilepsy: A relationship with seizure type, inflammation, and metabolic syndrome [J].

Kegler, Aline ;

Cardoso, Alexandra Seide ;

Fornari Caprara, Ana Leticia ;

Pascotini, Eduardo Tanuri ;

Arend, Josi ;

Gabbi, Patricia ;

Duarte, Marta M. M. F. ;

da Cruz, Ivana B. M. ;

Furian, Ana Flavia ;

Oliveira, Mauro Schneider ;

Freire Royes, Luiz Fernando ;

Fighera, Michele Rechia .

GENE, 2019, 711

[9] A novel antioxidant function for the tumor-suppressor gene p53 in the retinal ganglion cell [J].

O'Connor, Jeremy C. ;

Wallace, Deborah M. ;

O'Brien, Colm J. ;

Cotter, Thomas G. .

INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2008, 49 (10) :4237-4244

[10]

Robbins Delira, 2012, J Signal Transduct, V2012, P101465, DOI 10.1155/2012/101465

← 1 2 →