Identification of novel QTL associated with soybean isoflavone content

被引:42
作者
Pei, Ruili [1 ]
Zhang, Jingying [1 ]
Tian, Ling [1 ]
Zhang, Shengrui [1 ]
Han, Fenxia [1 ]
Yan, Shurong [1 ]
Wang, Lianzheng [1 ]
Li, Bin [1 ]
Sun, Junming [1 ]
机构
[1] Chinese Acad Agr Sci, Inst Crop Sci, MOA Key Lab Soybean Biol Beijing, Natl Engn Lab Crop Mol Breeding, Beijing 100081, Peoples R China
来源
CROP JOURNAL | 2018年 / 6卷 / 03期
基金
中国国家自然科学基金;
关键词
Soybean (Glycine max L. Merrill); QTL mapping; Isoflavones; Specific-locus amplified fragment sequencing (SLAF-seq); QUANTITATIVE TRAIT LOCI; BIOSYNTHESIS; PROLIFERATION; ACCUMULATION; EXPRESSION; GENISTEIN; SYNTHASE; PROTEIN; ENZYME; OIL;
D O I
10.1016/j.cj.2017.10.004
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Soybean isoflavones are essential secondary metabolites synthesized in the phenylpropanoid pathway and benefit human health. In the present study, high-resolution QTL mapping for isoflavone components was performed using specific-locus amplified fragment sequencing (SLAF-seq) with a recombinant inbred line (RIL) population (F-5: 7) derived from a cross between two cultivated soybean varieties, Luheidou 2 (LHD2) and Nanhuizao (NHZ). Using a high-density genetic map comprising 3541 SLAF markers and the isoflavone contents of soybean seeds in the 200 lines in four environments, 24 stable QTL were identified for isoflavone components, explaining 4.2%-21.2% of phenotypic variation. Of these QTL, four novel stable QTL (qG8, qMD19, qMG18, and qTIF19) were identified for genistin, malonyldaidzin, malonylgenistin, and total isoflavones, respectively. Gene annotation revealed three genes involved in isoflavone biosynthesis (Gm4CL, GmIFR, and GmCHR) and 13 MYB-like genes within genomic regions corresponding to stable QTL intervals, suggesting candidate genes underlying these loci. Nine epistatic QTL were identified for isoflavone components, explaining 4.7%-15.6% of phenotypic variation. These results will facilitate understanding the genetic basis of isoflavone accumulation in soybean seeds. The stable QTL and tightly linked SLAF markers may be used for markerassisted selection in soybean breeding programs. (C) 2018 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V.
引用
收藏
页码:244 / 252
页数:9
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