A near-infrared turn on fluorescent probe for biothiols detection and its application in living cells

被引：49

作者：

Yin, Caixia ^{[1
]}

Zhang, Weijie ^{[1
]}

Liu, Tao ^{[1
]}

Chao, Jianbin ^{[2
]}

Huo, Fangjun ^{[2
]}

机构：

[1] Shanxi Univ, Inst Mol Sci, Key Lab Mat Energy Convers & Storage Shanxi Prov, Key Lab Chem Biol & Mol Engn,Minist Educ, Taiyuan 030006, Peoples R China

[2] Shanxi Univ, Res Inst Appl Chem, Taiyuan 030006, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2017年 / 246卷

基金：

中国国家自然科学基金;

关键词：

Biothiols; Detection; NIR; Fluorescent probe; Bioimaging; IN-VIVO; HYDROGEN POLYSULFIDES; SELECTIVE DETECTION; GOLD NANOPARTICLES; CYANIDE PROBES; GLUTATHIONE; CYSTEINE; SENSORS; DESIGN; RED;

D O I：

10.1016/j.snb.2017.02.176

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Cysteine (Cys), homocysteine (Hcy) and Glutathione (GSH) are small-molecular biothiols that play key roles in various biological systems. The development of fluorescent probes for thiols has attracted intense interest. Inspired by the appealing advantages of near-infrared (NIR) fluorescent detection, we herein report a novel NIR fluorescent probe for biothiols detection based on the widely-used thiol-selective 2,4-dinitro-benzenesulfonyl (DNBS) group. The probe is intrinsically nonfluorescent; however, after being reacted with thiols, the DNBS group is substituted by the -SH group, which elicits a significant NIR fluorescent turn-on response. Probe displays 160-fold fluorescence intensity enhancement at 688 nm with 2 equiv of Cys in neutral buffer. More importantly, the probe features a good linearity range and the detection limit is as low as 63 nM. In addition, the probe uses long wavelength excitation (620 nm), all of such good properties proving it to be a good sensor for the selective detection of thiols and a potential use in biological applications. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：988 / 993

页数：6

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