Nesterenkonia cremea sp nov., a bacterium isolated from a soda lake

A Gram-stain-positive, aerobic, non-motile, rod-shaped, non-endospore-forming bacterial strain, 10C(T), was isolated from Lonar soda lake in India. Based on the 16S rRNA gene sequence analysis, this strain was identified as belonging to the genus Nesterenkonia and was most closely related to the type strains of Nesterenkonia lacusekhoensis (99.1 %, sequence similarity), Nesterenkonia aethiopica (96.9 %), Nesterenkonia flava (96.9 %) and related of the genus Nesterenkonia (< 96.6 %, sequence similarity). However, the DNA-DNA relatedness of strain 10C(T) with N. lacusekhoensis KCTC 19283 T was only 34.6 +/- 0.9. The DNA G+C content of strain 10C(T) was 68.6 mol%. Strain 10CT was an aerobic microbe with optimal growth at 37 degrees C, pH 7.5-8.0 and 5-6% (w/v) NaCl. The cell-wall peptidoglycan of strain 10C T was of the type A4a (L-Lys-L-Glu). The major polar lipids present were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. The major isoprenoid quinones were MK-7, MK-8 and MK-9. Major fatty acids of strain 10C(T) were anteiso-C-15 : 0, anteiso-C-17 : 0 and iso-C-16 : 0. The results of phylogenetic, chemotaxonomic and biochemical tests allowed a clear differentiation of strain 10C(T), which represents a novel member of the genus Nesterenkonia for which the name Nesterenkonia cremea sp. nov. is proposed. The type strain is 10C(T) (= LMG 29100(T) = KCTC 39636(T) = CGMCC 1.15388(T)).