Variegation and silencing in a lentiviral-based murine transgenic model

被引:16
作者
Baup, Delphine [1 ]
Fraga, Laurent [1 ]
Pernot, Eileen [2 ]
Van Acker, Annette [1 ]
Vanherck, Anne-Sophie [1 ]
Breckpot, Karine [3 ]
Thielemans, Kris [3 ]
Schurmans, Stephane [2 ]
Moser, Muriel [1 ]
Leo, Oberdan [1 ]
机构
[1] Univ Libre Bruxelles, Fac Sci, Physiol Anim Lab, Inst Biol & Med Mol, Gosselies, Belgium
[2] Univ Libre Bruxelles, Inst Rech Interdisciplinaire Biol Humaine & Mol, Fac Med, Gosselies, Belgium
[3] Vrije Univ Brussel, Sch Med, Dept Physiol & Immunol, Lab Mol & Cellular Therapy, Brussels, Belgium
关键词
Lentiviral transgenesis; Silencing; Variegation; RNA interference; Mice; VIVO GENE DELIVERY; RNA INTERFERENCE; IN-VIVO; MAMMALIAN-CELLS; DENDRITIC CELLS; STEM-CELLS; VECTORS; EXPRESSION; MICE; RATS;
D O I
10.1007/s11248-009-9318-4
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Lentiviral based constructs represent a recent development in the generation of transgenic animals. The ease of use, and the fact that the same backbone vectors can be used to down-modulate endogenous gene expression and to produce transgenic animals overexpressing a gene of interest, have fuelled growing interest in this technology. In this study, we have used a lentiviral delivery system to generate transgenic mice expressing altered levels (up or downregulated) of a gene of interest. Although this lentiviral-based approach led to high levels of transgenesis and germ line transmission, a wide variation in transgene expression was observed in most first and second generation mouse lines. In particular, despite the segregation of integrants into single-copy expressing mouse lines, transgene expression appeared to be the target of epigenetic regulatory mechanism, often causing the coexistence of high and low transgene expressing cells within a given tissue such as blood peripheral lymphocytes. The establishment and analysis of large number of mouse lines may therefore be required to select a stable transgenic line with pancellular expression of a gene of interest using this lentiviral-based approach.
引用
收藏
页码:399 / 414
页数:16
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