Fixation methods for electron microscopy of human and other liver

被引：78

作者：

Wisse, Eddie ^{[1
,2
,3
]}

Braet, Filip ^{[2
]}

Duimel, Hans ^{[1
]}

Vreuls, Celien ^{[1
]}

Koek, Ger ^{[3
]}

Damink, Steven W. M. Olde ^{[4
]}

van den Broek, Maartje A. J. ^{[4
]}

De Geest, Bart ^{[5
]}

Dejong, Cees H. C. ^{[4
]}

Tateno, Chise ^{[6
]}

Frederik, Peter ^{[1
]}

机构：

[1] Univ Maastricht, Electron Microscope Unit, NL-6200 MD Maastricht, Netherlands

[2] Univ Sydney, Australian Key Ctr Microscopy & Microanal, Sydney, NSW 2006, Australia

[3] Univ Maastricht, Dept Internal Med, NL-6200 MD Maastricht, Netherlands

[4] Univ Maastricht, Dept Surg, NL-6200 MD Maastricht, Netherlands

[5] Univ Leuven, Ctr Mol & Vasc Biol, B-3000 Louvain, Belgium

[6] PhoenixBio Co, Higashihiroshima 7390046, Japan

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2010年 / 16卷 / 23期

关键词：

Liver; Tissue fixation; Perfusion; Electron microscopy; Biopsy; PERFUSION-FIXATION; RAT-LIVER; ENDOTHELIAL FENESTRAE; SINUSOIDS; TRANSMISSION; SIZE; HEXAMETHYLDISILAZANE; IMMERSION; TISSUES; BIOPSY;

D O I：

10.3748/wjg.v16.i23.2851

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

For an electron microscopic study of the liver, expertise and complicated, time-consuming processing of hepatic tissues and cells is needed. The interpretation of electron microscopy (EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation, embedding, sectioning, contrast staining and microscopic imaging. Hence, the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue, for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects. (C) 2010 Baishideng. All rights reserved.

引用

页码：2851 / 2866

页数：16

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