Modified chitosan-based bioactive material for antimicrobial application: Synthesis and characterization

被引：59

作者：

de Araujo Braz, Elton Marks ^{[1
]}

Cavalcante Costa e Silva, Solranny Carla ^{[1
]}

da Silva, Durcilene Alves ^{[1
]}

de Amorim Carvalho, Fernando Aecio ^{[2
]}

Barreto, Humberto Medeiros ^{[3
]}

Santos Junior, Luiz de Sousa ^{[1
]}

da Silva Filho, Edson Cavalcanti ^{[1
]}

机构：

[1] Univ Fed Piaui, Lab Interdisciplinar Mat Avancados LIMAV, Campus Minist Petronio Portela, BR-64049550 Teresina, PI, Brazil

[2] Univ Fed Piaui, NPPM, Campus Minist Petronio Portela, BR-64049550 Teresina, PI, Brazil

[3] Univ Fed Piaui, Lab Pesquisa Microbiol, Campus Univ Minist Petronio Portella, BR-64049550 Teresina, PI, Brazil

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2018年 / 117卷

关键词：

Grafted chitosan; Chemical modification; Anhydride cytotoxicity; Antimicrobial activity; HYDROPHOBICALLY-MODIFIED CHITOSAN; IN-VITRO EVALUATION; ANTIBACTERIAL ACTIVITY; ESSENTIAL OIL; BIOCOMPATIBILITY; NANOPARTICLES; MEMBRANES;

D O I：

10.1016/j.ijbiomac.2018.05.205

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The search for natural antibacterial agents to treat diseases caused by resistant microorganisms has been gaining increasing attention. Chitosan has been studied in several areas due to its particular properties. The grafting of hydrophobic chains into the chitosan molecule, turning it amphiphilic, may improve its antimicrobial activity by increasing electrostatic interaction with the bacterial cell wall. The objective of this work was to enhance the antimicrobial activity of chitosan by the reaction of N-acylation with maleic anhydride. For this purpose, molar ratios of 1:2, 1:5 and 1:10 chitosan: anhydride were investigated, and the obtained derivatives were characterized by elemental analysis, FTIR, thermal analysis and XRD where it was possible to prove the chemical modification of chitosan. The modified materials presented excellent antibacterial action against Staphylococcus aureus and Escherichia coli, evidencing no activity against the protozoan Leishmania amazonensis. Cytotoxicity assays by the MTT analysis and hemolysis indicated that the derivatives did not show toxicity in mammalian cells. The proposed modified chitosan compounds showed to be promising for biomedical applications since they allied excellent antibacterial activity and absence of cytotoxicity. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：640 / 647

页数：8

共 38 条

[21] Syntheses of novel chitosan derivative with excellent solubility, anticoagulation, and antibacterial property by chemical modification [J].

Liu, Hengquan ;

Zhao, Yuancong ;

Cheng, Si ;

Huang, Nan ;

Leng, Yongxiang .

JOURNAL OF APPLIED POLYMER SCIENCE, 2012, 124 (04) :2641-2648

[22] Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPS) isolated from aquatic animals [J].

Loefgren, S. E. ;

Miletti, L. C. ;

Steindel, M. ;

Bachere, E. ;

Barracco, M. A. .

EXPERIMENTAL PARASITOLOGY, 2008, 118 (02) :197-202

[23]

Mahae N., 2011, INT FOOD RES J, V18

[24] Engineering hydrophobically modified chitosan for enhancing the dispersion of respirable microparticles of levofloxacin [J].

Merchant, Zahra ;

Taylor, Kevin M. G. ;

Stapleton, Paul ;

Razak, Sana A. ;

Kunda, Nitesh ;

Alfagih, Iman ;

Sheikh, Khalid ;

Saleem, Imran Y. ;

Somavarapu, Satyanarayana .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2014, 88 (03) :816-829

[25] Improving chitosan-mediated gene transfer by the introduction of intracellular buffering moieties into the chitosan backbone [J].

Moreira, C. ;

Oliveira, H. ;

Pires, L. R. ;

Simoes, S. ;

Barbosa, M. A. ;

Pego, A. P. .

ACTA BIOMATERIALIA, 2009, 5 (08) :2995-3006

[26]

Nogueira L., 2007, REV ELETRONICA FARMA, V4

[27] Teichoic acid biosynthesis as an antibiotic target [J].

Pasquina, Lincoln W. ;

Maria, John P. Santa ;

Walker, Suzanne .

CURRENT OPINION IN MICROBIOLOGY, 2013, 16 (05) :531-537

[28] Enzymatic degradation and bioactivity evaluation of C-6 oxidized chitosan [J].

Pierre, Guillaume ;

Salah, Rym ;

Gardarin, Christine ;

Traikia, Mounir ;

Petit, Emmanuel ;

Delort, Anne-Marie ;

Mameri, Nabil ;

Moulti-Mati, Farida ;

Michaud, Philippe .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2013, 60 :383-392

[29] In vitro evaluation of the effectiveness and cytotoxicity of meglumine antimoniate microspheres produced by spray drying against Leishmania infantum [J].

Pujals, G. ;

Sune-Negre, J. M. ;

Perez, P. ;

Garcia, E. ;

Portus, M. ;

Tico, J. R. ;

Minarro, M. ;

Carrio, J. .

PARASITOLOGY RESEARCH, 2008, 102 (06) :1243-1247

[30] Chitosan-based nanomaterials: A state-of-the-art review [J].

Shukla, Sudheesh K. ;

Mishra, Ajay K. ;

Arotiba, Omotayo A. ;

Mamba, Bhekie B. .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2013, 59 :46-58

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