A culture medium for screening 16S rRNA methylase-producing pan-aminoglycoside resistant Gram-negative bacteria

被引:6
|
作者
Nordmann, Patrice [1 ,2 ,3 ,4 ,5 ,6 ]
Maze, Alain [1 ,2 ,3 ]
Culebras, Esther [7 ]
Dobias, Jan [1 ,2 ,3 ]
Jayol, Aurelie [1 ,2 ,3 ]
Poirel, Laurent [1 ,2 ,3 ]
机构
[1] Univ Fribourg, Fac Sci, Dept Med, Med & Mol Microbiol Unit, Fribourg, Switzerland
[2] Univ Fribourg, Fribourg, Switzerland
[3] IAME, LEA, INSERM European Unit, Paris, France
[4] Swiss Natl Reference Ctr Emerging Antibiot Resist, Fribourg, Switzerland
[5] Univ Lausanne, Inst Microbiol, Lausanne, Switzerland
[6] Univ Hosp Ctr, Lausanne, Switzerland
[7] Hosp Clin San Carlos, Microbiol Dept, Madrid, Spain
关键词
16S rRNA methylases; Antibiotic; Susceptibility testing; Gentamicin; Amikacin; Tobramycin; Netilmicin; Kanamycin; Plazomicin; ESCHERICHIA-COLI; METHYLTRANSFERASE;
D O I
10.1016/j.diagmicrobio.2018.01.026
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
The amikacin plus gentamicin-containing Super Aminoglycoside medium was developed for screening multipleaminoglycoside resistance in Gram-negative bacteria (Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii). It was evaluated using aminoglycoside-susceptible (n=12) and aminoglycoside-resistant (n=59) Gram-negative isolates, including 16S rRNA methylase producers (n=20). Its sensitivity and specificity of detection were, respectively, of 95% and 96% for detecting multiple aminoglycoside-resistant methylase producers. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码:118 / 122
页数:5
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