siRNAs encapsulated in recombinant capsid protein derived from Dengue serotype 2 virus inhibits the four serotypes of the virus and proliferation of cancer cells

被引：6

作者：

Kumar, A. S. Manoj ^{[1
]}

Reddy, G. E. C. Vidyadhar ^{[1
]}

Rajmane, Yogesh ^{[1
]}

Nair, Soumya ^{[1
]}

Kamath, Sangita Pai ^{[1
]}

Sreejesh, Greeshma ^{[1
]}

Basha, Khalander ^{[1
]}

Chile, Shailaja ^{[1
]}

Ray, Kriti ^{[1
]}

Nelly, Vivant ^{[2
]}

Khadpe, Nilesh ^{[2
]}

Kasturi, Ravishankar ^{[2
]}

Ramana, Venkata ^{[1
]}

机构：

[1] Dhirubhai Ambani Life Sci Ctr, Therapeut Prot Mol Biol Grp, Navi Mumbai 400701, Maharashtra, India

[2] Dhirubhai Ambani Life Sci Ctr, Therapeut Prot Proc Dev Grp, Navi Mumbai 400701, Maharashtra, India

来源：

JOURNAL OF BIOTECHNOLOGY | 2015年 / 193卷

关键词：

siRNA; Dengue; Capsid; Aurorakinase; Rho A; NUCLEOCAPSID-LIKE PARTICLES; SMALL INTERFERING RNA; IN-VITRO; MOUSE MODELS; AG129; MICE; REPLICATION; DELIVERY; DISEASE; THERAPEUTICS; MACROPHAGES;

D O I：

10.1016/j.jbiotec.2014.11.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

siRNA delivery potential of the Dengue virus capsid protein in cultured cells was recently reported, but target knockdown potential in the context of specific diseases has not been explored. In this study we have evaluated the utility of the protein as an siRNA carrier for anti Dengue viral and anti cancer applications using cell culture systems. We show that target specific siRNAs delivered using the capsid protein inhibit infection by the four serotypes of Dengue virus and proliferation of two cancer cell lines. Our data confirm the potential of the capsid for anti Dengue viral and anti cancer RNAi applications. In addition, we have optimized a fermentation strategy to improve the yield of Escherichia coli expressed D2C protein since the reported yields of E. coli expressed flaviviral capsid proteins are low. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：23 / 33

页数：11