Tegumental changes in adult Schistosoma mansoni harboured in mice treated with praziquantel enantiomers

被引:23
作者
Xiao, SH
Shen, BG
Collet, J
Tanner, M
机构
[1] Swiss Trop Inst, CH-4002 Basel, Switzerland
[2] Chinese Acad Prevent Med, Inst Parasit Dis, Shanghai 200025, Peoples R China
关键词
schistosomiasis; Schistosoma mansoni; praziquantel; enantiomers; scanning electron microscopy;
D O I
10.1016/S0001-706X(00)00076-0
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
Praziquantel administered to the host causes damage to the tegument of Schistosoma mansoni. In this study, the effects of racemic praziquantel (Pra) and its enantiomers, levo-praziquantel (r-Pra) and dextro-praziquantel (D-Pra) were compared using scanning electron microscopy (SEM). Mice infected with S. mansoni for 49 days were treated with a single dose of Pra (300 mg/kg), L-Pra (150 mg/kg) or D-Pra (150 or 600 mg/kg). Groups of three mice were killed after 4 and 24 h, and schistosomes collected by perfusion and examined by SEM. Treatment with Pra or r-Pra, for 4 or 24 h, caused tegumental damage to S. mansoni including severe swelling, vacuolization,fusion of the tegumental ridges and loss or shortening of the spines on the tubercles, collapse and peeling. After treatment with D-Pra at 150 mg/kg, no apparent damage was observed. When the dosage was increased to 600 mg/kg, after 4 h lesions on the tegument similar to those induced by Pra or L-Pra were seen, but less severe. After 24 h, there was evidence of recovery. The study thus clearly showed that r-Pra was more active than D-Pra in causing tegumental damage. D-Pra showed a qualitatively similar activity at a higher concentration. It is possible that this effect was due at least to some extent to the small amount of r-Pra (< 2%) which was present in the preparation of D-Pra used. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:107 / 117
页数:11
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