Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo

被引：114

作者：

Hasuwa, Hidetoshi ^{[1
]}

Muro, Yuko ^{[1
]}

Ikawa, Masahito ^{[1
]}

Kato, Noriko ^{[2
]}

Tsujimoto, Yoshihide ^{[2
]}

Okabe, Masaru ^{[1
]}

机构：

[1] Osaka Univ, Genome Informat Res Ctr, Microbial Dis Res Inst, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Genet Mol Lab, Dept Med Genet, Sch Med, Suita, Osaka 5650871, Japan

来源：

EXPERIMENTAL ANIMALS | 2010年 / 59卷 / 01期

关键词：

live imaging; oviduct; sperm; TRANSPORT; OVIDUCT; GFP;

D O I：

10.1538/expanim.59.105

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.

引用

页码：105 / 107

页数：3

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