Effects of 1, 25-Dihydroxyvitamin D3 on Experimental Autoimmune Myocarditis in Mice

被引:19
作者
Hu, Fen [1 ]
Yan, Lianhua [1 ]
Lu, Shuai [1 ]
Ma, Wenhan [1 ]
Wang, Ya [1 ]
Wei, Yuzhen [1 ]
Yan, Xiaofei [1 ]
Zhao, Xin [1 ]
Chen, Zhijian [1 ]
Wang, Zhaohui [1 ]
Cheng, Bo [2 ]
机构
[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Lab Cardiovasc Immunol,Inst Cardiol, 1277 Jiefang Ave, Wuhan 430022, Hubei, Peoples R China
[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Dept Stomatol, 1277 Jiefang Ave, Wuhan 430022, Hubei, Peoples R China
基金
中国国家自然科学基金;
关键词
Experimental autoimmune myocarditis; 1; 25(OH)2; D3; Apoptosis; Autophagy; VITAMIN-D; AUTOPHAGY CONTRIBUTES; ENDOTHELIAL-CELLS; VIRAL MYOCARDITIS; CANCER-RISK; ACTIVATION; DISEASE; DEATH; RATS; METAANALYSIS;
D O I
10.1159/000445577
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Background/Aims: Myocarditis is an important inflammatory disease of the heart which causes life-threatening conditions. 1, 25(OH)2 D3 has effects on multiple systems and diseases. The present study was aimed to investigate the effect of 1, 25(OH)2 D3 on experimental autoimmune myocarditis (EAM), and explored the underlying mechanisms involved. Methods: EAM was induced by immunizing BALB/c mice with cardiac alpha-myosin heavy chain peptides (MyHC-alpha). 1, 25(OH)2 D3 (1,000 ng/kg once) or vehicle was administered intraperitoneally every other day during the entire experiment. On day 21, transthoracic echocardiography was performed and cardiac inflammatory infiltration was detected by hematoxylin and eosin (HE). The terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) assay, and Western blots for the expression of protein caspase-3 and cleaved-caspase3 were used to evaluate apoptosis. Transmission electron microscopy and Western blots for the expression of protein Beclin-1, LC3B, and P62 were used to evaluate autophagy. Results: The ratio of heart weight/body weight was significantly reduced in 1, 25(OH)2 D3-treated EAM mice, compared with vehicle-treated ones. 1, 25(OH)2 D3 treatment improved cardiac function, diminished cell infiltration in cardiac, suppressed myocardial apoptosis, decreased the number of autophagosomes, and decreased the protein expression of Beclin-1, LC3-II and p62. Conclusions: The present results demonstrated that administration of 1, 25(OH)2 D3 decreased EAM severity. 1, 25(OH)2 D3 treatment may be a feasible therapeutic approach for EAM. (C) 2016 The Author(s) Published by S. Karger AG, Basel
引用
收藏
页码:2219 / 2229
页数:11
相关论文
共 47 条
  • [1] Control of autoimmune diseases by the vitamin D endocrine system
    Adorini, Luciano
    Penna, Giuseppe
    [J]. NATURE CLINICAL PRACTICE RHEUMATOLOGY, 2008, 4 (08): : 404 - 412
  • [2] Vitamin D and cardiovascular disease: is the evidence solid?
    Al Mheid, Ibhar
    Patel, Riyaz S.
    Tangpricha, Vin
    Quyyumi, Arshed A.
    [J]. EUROPEAN HEART JOURNAL, 2013, 34 (48) : 3691 - 3698
  • [3] Association between 25-hydroxyvitamin D concentration and breast cancer risk in an Australian population: an observational case-control study
    Bilinski, K.
    Boyages, J.
    [J]. BREAST CANCER RESEARCH AND TREATMENT, 2013, 137 (02) : 599 - 607
  • [4] Immune-mediated and autoimmune myocarditis: clinical presentation, diagnosis and management
    Caforio, Alida L. P.
    Marcolongo, Renzo
    Jahns, Roland
    Fu, Michael
    Felix, Stephan B.
    Iliceto, S.
    [J]. HEART FAILURE REVIEWS, 2013, 18 (06) : 715 - 732
  • [5] miR-145 mediates the antiproliferative and gene regulatory effects of vitamin D3 by directly targeting E2F3 in gastric cancer cells
    Chang, Su'e
    Gao, Ling
    Yang, Yang
    Tong, Dongdong
    Guo, Bo
    Liu, Liying
    Li, Zongfang
    Song, Tusheng
    Huang, Chen
    [J]. ONCOTARGET, 2015, 6 (10) : 7675 - 7685
  • [6] 1,25-Dihydroxyvitamin D3 inhibits lipopolysaccharide-induced immune activation in human endothelial cells
    Equils, O
    Naiki, Y
    Shapiro, AM
    Michelsen, K
    Lu, D
    Adams, J
    Jordan, S
    [J]. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2006, 143 (01) : 58 - 64
  • [7] Liver autophagy contributes to the maintenance of blood glucose and amino acid levels
    Ezaki, Junji
    Matsumoto, Naomi
    Takeda-Ezaki, Mitsue
    Komatsu, Masaaki
    Takahashi, Katsuyuki
    Hiraoka, Yuka
    Taka, Hikari
    Fujimura, Tsutomu
    Takehana, Kenji
    Yoshida, Mitsutaka
    Iwata, Junichi
    Tanida, Isei
    Furuya, Norihiko
    Zheng, Dong-Mei
    Tada, Norihiro
    Tanaka, Keiji
    Kominami, Eiki
    Ueno, Takashi
    [J]. AUTOPHAGY, 2011, 7 (07) : 727 - 736
  • [8] The role of vitamin D in reducing cancer risk and progression
    Feldman, David
    Krishnan, Aruna V.
    Swami, Srilatha
    Giovannucci, Edward
    Feldman, Brian J.
    [J]. NATURE REVIEWS CANCER, 2014, 14 (05) : 342 - 357
  • [9] Vitamin D3 Induces Tolerance in Human Dendritic Cells by Activation of Intracellular Metabolic Pathways
    Ferreira, Gabriela Bomfim
    Vanherwegen, An-Sofie
    Eelen, Guy
    Gutierrez, Ana Carolina Fierro
    Van Lommel, Leentje
    Marchal, Kathleen
    Verlinden, Lieve
    Verstuyf, Annemieke
    Nogueira, Tatiane
    Georgiadou, Maria
    Schuit, Frans
    Eizirik, Decio L.
    Gysemans, Conny
    Carmeliet, Peter
    Overbergh, Lut
    Mathieu, Chantal
    [J]. CELL REPORTS, 2015, 10 (05): : 711 - 725
  • [10] Cell death modalities: classification and pathophysiological implications
    Galluzzi, L.
    Maiuri, M. C.
    Vitale, I.
    Zischka, H.
    Castedo, M.
    Zitvogel, L.
    Kroemer, G.
    [J]. CELL DEATH AND DIFFERENTIATION, 2007, 14 (07) : 1237 - 1243