Development of Linum flavum hairy root cultures for production of coniferin

被引：36
|
作者：
Lin, HW
Kwok, KH
Doran, PM [1 ]
机构：
[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia
[2] Tridan Ltd Albright & Wilson Australia Ltd, Cooperat Res Ctr Bioprod, Yarraville, Vic 3013, Australia
来源：
BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 07期
关键词：
coniferin; hairy root; Linum flavum;
D O I：
10.1023/A:1022821600283
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Linum flavum hairy roots were initiated from leaf discs using Agrobacterium rhizogenes strains LBA9402 and TR105 though two other strains, 15834 and A4, were relatively ineffective for induction. Significant variation in coniferin accumulation was observed between hairy root lines originating from different L. flavum seedlings and/or A. rhizogenes strains. Coniferin reached 58 mg g(-1) dry wt by culturing the roots in Linsmaier and Skoog (LS) medium with 2,4-dichlorophenoxyacetic acid and naphthaleneacetic acid as growth regulators.
引用
收藏
页码：521 / 525
页数：5
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