Development of 11 microsatellite loci for population studies in the swordfish, Xiphias gladius (Teleostei: Scombridae)

被引:13
作者
Reeb, CA [1 ]
Arcangeli, L [1 ]
Block, BA [1 ]
机构
[1] Stanford Univ, Dept Biol Sci, Hopkins Marine Stn, Pacific Grove, CA 93950 USA
来源
MOLECULAR ECOLOGY NOTES | 2003年 / 3卷 / 01期
关键词
microsatellites; swordfish; Xiphias gladius;
D O I
10.1046/j.1471-8286.2003.00385.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Eleven microsatellite loci were developed in the swordfish, Xiphias gladius, to examine population structure. Loci were amplified in samples from two geographically distant populations (Ecuador and Mediterranean Sea). Observed heterozygosity ranged from 0.150 to 0.960. Most loci conformed to Hardy-Weinberg expectations with the exception of locus Xg-59, which had a heterozygote deficit caused probably by null alleles. Locus Xg-402 did not show significant divergence between the populations. The remaining nine loci showed statistically significant spatial heterogeneity (genic differentiation) between the two populations, making them useful for future studies of population structure in swordfish.
引用
收藏
页码:147 / 149
页数:3
相关论文
共 7 条
[1]   MITOCHONDRIAL-DNA CONTROL REGION SEQUENCES INDICATE EXTENSIVE MIXING OF SWORDFISH (XIPHIAS-GLADIUS) POPULATIONS IN THE ATLANTIC-OCEAN [J].
BREMER, JRA ;
BAKER, AJ ;
MEJUTO, J .
CANADIAN JOURNAL OF FISHERIES AND AQUATIC SCIENCES, 1995, 52 (08) :1720-1732
[2]   Genetic stock structure of the swordfish (Xiphias gladius) inferred by PCR-RFLP analysis of the mitochondrial DNA control region [J].
Chow, S ;
Okamoto, H ;
Uozumi, Y ;
Takeuchi, Y ;
Takeyama, H .
MARINE BIOLOGY, 1997, 127 (03) :359-367
[3]  
Hecker KH, 1996, BIOTECHNIQUES, V20, P478
[4]   NONAMPLIFYING ALLELES AT MICROSATELLITE LOCI - A CAUTION FOR PARENTAGE AND POPULATION STUDIES [J].
PEMBERTON, JM ;
SLATE, J ;
BANCROFT, DR ;
BARRETT, JA .
MOLECULAR ECOLOGY, 1995, 4 (02) :249-252
[5]   GENEPOP (VERSION-1.2) - POPULATION-GENETICS SOFTWARE FOR EXACT TESTS AND ECUMENICISM [J].
RAYMOND, M ;
ROUSSET, F .
JOURNAL OF HEREDITY, 1995, 86 (03) :248-249
[6]   Structure and migration corridors in Pacific populations of the Swordfish Xiphius gladius, as inferred through analyses of mitochondrial DNA [J].
Reeb, CA ;
Arcangeli, L ;
Block, BA .
MARINE BIOLOGY, 2000, 136 (06) :1123-1131
[7]   Mitochondrial control region variability and global population structure in the swordfish, Xiphias gladius [J].
Rosel, PE ;
Block, BA .
MARINE BIOLOGY, 1996, 125 (01) :11-22