Elevation of intracellular calcium induced by the intrapipette perfusion technique modifies membrane ion currents in the chick cochlear hair cell

被引：0

作者：

Kimitsuki, T ^{[1
]}

Nakagawa, T ^{[1
]}

Hisashi, K ^{[1
]}

Tsuji, K ^{[1
]}

Komune, S ^{[1
]}

Komiyama, S ^{[1
]}

机构：

[1] Kyushu Univ, Fac Med, Dept Otorhinolaryngol, Higashi Ku, Fukuoka 81282, Japan

来源：

ACTA OTO-LARYNGOLOGICA | 1998年 / 118卷 / 01期

关键词：

hair cell; patch-clamp; intrapipette perfusion; Ca2+-activated K+ current;

D O I：

10.1080/00016489850155152

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

The concentration of intracellular calcium ion ([Ca2+](i)) was altered in the same hair cell dissociated from a chick cochlea by the intrapipette perfusion technique. At a membrane potential of -40 mV, the elevation of [Ca2+](i) generated outward-going currents within 60 sec when the intrapipette solution was based on KCl. In controls, at membrane potentials more positive than -50 mV, outward K+ currents were observed and at large positive potentials, the outward K+ current decreased, showing an N-shaped I-V relationship. This outward K+ current was increased by elevation of [Ca2+], and was partially suppressed by a TEA-containing extracellular solution. We suggest that the Ca2+ increased by the intrapipette perfusion technique operates directly inside the cell membrane and activates Ca2+-activated K+ currents.

引用

页码：70 / 73

页数：4