High-Throughput and Cost-Effective Characterization of Induced Pluripotent Stem Cells

被引:57
作者
D'Antonio, Matteo [1 ]
Woodruff, Grace [2 ]
Nathanson, Jason L. [2 ]
D'Antonio-Chronowska, Agnieszka [3 ]
Arias, Angelo [1 ]
Matsui, Hiroko [3 ]
Williams, Roy [3 ]
Herrera, Cheryl [2 ]
Reyna, Sol M. [2 ]
Yeo, Gene W. [2 ,3 ]
Goldstein, Lawrence S. B. [2 ,4 ]
Panopoulos, Athanasia D. [5 ]
Frazer, Kelly A. [1 ,3 ]
机构
[1] Univ Calif San Diego, Dept Pediat, La Jolla, CA 92093 USA
[2] Univ Calif San Diego, Dept Cellular & Mol Med, La Jolla, CA 92093 USA
[3] Univ Calif San Diego, Inst Genom Med, La Jolla, CA 92093 USA
[4] Univ Calif San Diego, Dept Neurosci, La Jolla, CA 92093 USA
[5] Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA
关键词
COPY NUMBER; ABNORMALITIES;
D O I
10.1016/j.stemcr.2017.03.011
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Reprogramming somatic cells to induced pluripotent stem cells (iPSCs) offers the possibility of studying the molecular mechanisms underlying human diseases in cell types difficult to extract from living patients, such as neurons and cardiomyocytes. To date, studies have been published that use small panels of iPSC-derived cell lines to study monogenic diseases. However, to study complex diseases, where the genetic variation underlying the disorder is unknown, a sizable number of patient-specific iPSC lines and controls need to be generated. Currently the methods for deriving and characterizing iPSCs are time consuming, expensive, and, in some cases, descriptive but not quantitative. Here we set out to develop a set of simple methods that reduce cost and increase throughput in the characterization of iPSC lines. Specifically, we outline methods for high-throughput quantification of surface markers, gene expression analysis of in vitro differentiation potential, and evaluation of karyotype with markedly reduced cost.
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收藏
页码:1101 / 1111
页数:11
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