Serotyping Pneumococcal Meningitis Cases in the African Meningitis Belt by Use of Multiplex PCR with Cerebrospinal Fluid

被引：19

作者：

Lafourcade, Berthe-Marie Njanpop ^{[1
]}

Sanou, Oumarou ^{[2
]}

van der Linden, Mark ^{[3
]}

Levina, Natalia ^{[3
]}

Karanfil, Meryem ^{[3
]}

Yaro, Seydou

Tamekloe, Tsidi A. ^{[4
]}

Mueller, Judith E. ^{[1
]}

机构：

[1] Inst Pasteur, Agence Med Prevent, F-75724 Paris 15, France

[2] Ctr Muraz, Agence Med Prevent, Bobo Dioulasso 01, Burkina Faso

[3] Univ Hosp RWTH Aachen, Dept Med Microbiol, German Natl Reference Ctr Streptococci, D-52074 Aachen, Germany

[4] Minist Hlth Togo, Div Epidemiol, Lome, Togo

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2010年 / 48卷 / 02期

关键词：

DETERMINING CAPSULAR SEROTYPES; NEISSERIA-MENINGITIDIS; BURKINA-FASO; STREPTOCOCCUS-PNEUMONIAE; EPIDEMIC; DISEASE;

D O I：

10.1128/JCM.01402-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We reformulated a multiplex PCR algorithm for serotyping of pneumococcal meningitis directly on cerebrospinal fluid (CSF). Compared to established methods on isolates, CSF-based PCR had at least 80% sensitivity and 100% specificity. In regional meningitis surveillance, CSF-based PCR increased the serotype information yield from 40% of cases (isolate testing) to 90%.

引用

页码：612 / 614

页数：3

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