Serotyping Pneumococcal Meningitis Cases in the African Meningitis Belt by Use of Multiplex PCR with Cerebrospinal Fluid

被引:19
|
作者
Lafourcade, Berthe-Marie Njanpop [1 ]
Sanou, Oumarou [2 ]
van der Linden, Mark [3 ]
Levina, Natalia [3 ]
Karanfil, Meryem [3 ]
Yaro, Seydou
Tamekloe, Tsidi A. [4 ]
Mueller, Judith E. [1 ]
机构
[1] Inst Pasteur, Agence Med Prevent, F-75724 Paris 15, France
[2] Ctr Muraz, Agence Med Prevent, Bobo Dioulasso 01, Burkina Faso
[3] Univ Hosp RWTH Aachen, Dept Med Microbiol, German Natl Reference Ctr Streptococci, D-52074 Aachen, Germany
[4] Minist Hlth Togo, Div Epidemiol, Lome, Togo
关键词
DETERMINING CAPSULAR SEROTYPES; NEISSERIA-MENINGITIDIS; BURKINA-FASO; STREPTOCOCCUS-PNEUMONIAE; EPIDEMIC; DISEASE;
D O I
10.1128/JCM.01402-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
We reformulated a multiplex PCR algorithm for serotyping of pneumococcal meningitis directly on cerebrospinal fluid (CSF). Compared to established methods on isolates, CSF-based PCR had at least 80% sensitivity and 100% specificity. In regional meningitis surveillance, CSF-based PCR increased the serotype information yield from 40% of cases (isolate testing) to 90%.
引用
收藏
页码:612 / 614
页数:3
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