Zhiheshouwu ethanol extract induces intrinsic apoptosis and reduces unsaturated fatty acids via SREBP1 pathway in hepatocellular carcinoma cells

被引:17
作者
Li, Hongliang [1 ,2 ]
Xiang, Longchao [1 ,2 ]
Yang, Nian [3 ]
Cao, Fengjun [1 ]
Li, Chen [1 ,2 ,5 ]
Chen, Ping [1 ]
Ruan, Xuzhi [2 ,5 ]
Feng, Yibin [4 ]
Zhou, Nian [1 ,2 ]
Wang, Xuanbin [1 ,2 ]
机构
[1] Hubei Univ Med, Renmin Hosp, Ctr Oncol, Lab Chinese Herbal Pharmacol, 39 Middle Chaoyang Rd, Shiyan 442000, Hubei, Peoples R China
[2] Hubei Univ Med, Sch Pharm, Dept Pharmacol, Hubei Key Lab Wudang Local Chinese Med Res, 30 South Renmin Rd, Shiyan 442000, Hubei, Peoples R China
[3] Hubei Univ Sci & Technol, Xianning 437100, Hubei, Peoples R China
[4] Univ Hong Kong, Sch Chinese Med, 10 Sassoon Rd, Pokfulam, Hong Kong, Peoples R China
[5] Hubei Univ Med, Lab Med Plant, Sch Basic Med, Shiyan 442000, Peoples R China
基金
中国国家自然科学基金;
关键词
Zhiheshouwu ethanol extract; Hepatocellular carcinoma; Intrinsic apoptosis; SREBP1; INDUCED MITOCHONDRIAL DYSFUNCTION; STEAROYL-COA DESATURASE-1; SURVIVAL; CANCER; INHIBITION;
D O I
10.1016/j.fct.2018.04.054
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Hepatocellular carcinoma (HCC) is the major incidence and one of the most life-threatening cancer. How to conquer HCC is a worldwide issue for patients. Zhiheshouwu (Polygon(multiflori Radix Praeparata) is a Chinese medicinal herb exhibiting both lowering lipid and inhibiting cancer cells. However, it remains a matter if its inhibiting cancer cells is related to its lowering lipid. In this study, we investigate the effects of Zhiheshouwu ethanolic extract (HSWE) on apoptosis and the underlying mechanisms in Bel-7402 cells. The results showed that HSWE inhibited the proliferation with an increased level of ALT and AST in Bel-7402 cells. The decreased mitochondrial membrane potential (WPM) was observed in HSWE-treated Bel-7402 cells. The flow cytometry results showed that HSWE triggered apoptosis. Since mitochondrial injury is characterized as intrinsic apoptotic cell death, these data indicated that HSWE may induce intrinsic apoptosis in Bel-7402 cells. In addition, HSWE decreased the production of unsaturated fatty acids, and inhibited the mRNA and protein of SCD1 and its upstream factor, sterol-regulatory element binding proteins 1 (SREBP1), a master transcriptional regulator of lipogenic gene. Taken together, these data suggest that HSWE induces an intrinsic apoptosis, and reduced unsaturated fatty acids by blocking SREBP1 in hepatocellular carcinoma cells.
引用
收藏
页码:169 / 175
页数:7
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