Intracellularly grown gold nanoparticles as potential surface-enhanced Raman scattering probes

被引：75
作者：
Shamsaie, Ali
Jonczyk, Magdalena
Sturgis, Jennie
Robinson, J. Paul
Irudayaraj, Joseph
机构：
[1] Purdue Univ, Dept Agr & Biol Engn, W Lafayette, IN 47907 USA
[2] Bindley Biosci Ctr, W Lafayette, IN 47907 USA
来源：
JOURNAL OF BIOMEDICAL OPTICS | 2007年 / 12卷 / 02期
关键词：
surface-enhanced Raman scattering; confocal Raman microscopy; confocal reflectance microscopy; gold formation; silver enhancement; intracellular growth;
D O I：
10.1117/1.2717549
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Gold nanoparticles grown within the intracellular confines of living cells are introduced as potential surface-enhanced Raman scattering (SERS) substrates for confocal Raman spectrometry. Electron microscopy and a silver-enhanced reflectance laser scanning confocal microscopic approach were used to visualize the size, shape, and distribution of intracellularly grown gold nanoparticles (IGAuN) as small as 1 nm. Passive uptake as the conventional approach for delivering nanoparticles inside cells faces the insurmountable challenge of escaping the endosomal/lysosomal pathway. In contrast, IGAuN provides an unprecedented advantage of providing access to cytoplasm and nucleus. (C) 2007 Society of Photo-Optical Instrumentation Engineers.
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