In vitro synergy of azole antifungals and methotrexate against Candida albicans

被引:9
作者
Yang, Jianxun [1 ]
Gao, Lei [2 ]
Yu, Pei [1 ]
Kosgey, Janet Cheruiyot [3 ]
Jia, Lina [3 ]
Fang, Yong [3 ]
Xiong, Jikui [1 ]
Zhang, Fengmin [3 ]
机构
[1] Harbin Med Univ, Hosp 2, Dept Dermatol, Harbin 150080, Heilongjiang, Peoples R China
[2] Westlake Univ, Microscopy Core Facil, Hangzhou 310024, Zhejiang, Peoples R China
[3] Harbin Med Univ, WU Lien Inst, Dept Microbiol, Harbin 150080, Heilongjiang, Peoples R China
基金
中国国家自然科学基金;
关键词
Methotrexate; Azole antifungal; Candida albicans; Synergy; INVASIVE CANDIDIASIS; EPIDEMIOLOGY; FLUCONAZOLE; ANTIBIOFILM; MANAGEMENT; TRANSPLANT; GROWTH; RISK;
D O I
10.1016/j.lfs.2019.116827
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Objective: This study aims to evaluate the effective of azoles and MTX for patients with invasive candidiasis. Methods: We used the disk diffusion assay and the checkerboard assay to evaluate the in vitro interactions between MTX and antifungals. In addition, we used the transmission electron microscopy to observe the ultrastructure of the effect of MTX and fluconazole on Candida albicans. Results: The rates of synergy for the combination of MTX with fluconazole (FLC), itraconazole (ITC), and voriconazole (VRZ) were 91.3%, 65.2%, and 87% in checkerboard testing. No antagonism was found between methotrexate and azole antifungals in any of the strains. Furthermore, MTX treated C. albicans showed extensive cell wall vacuolations and the inhibition of blastospores growth, as observed using transmission electron microscopy. There was an apparent destruction of the cell membrane and cell wall resulting in the destruction of cytoplasm, a phenomenon observed when MTX was combined with azoles. Conclusion: This study provides evidence that the combination of azoles and MTX is effective for patients with invasive candidiasis, which on the other hand, will reduce the side effects of the drugs.
引用
收藏
页数:5
相关论文
共 21 条
[1]   Biopolymer gelatin-coated zinc oxide nanoparticles showed high antibacterial, antibiofilm and anti-angiogenic activity [J].
Divya, Mani ;
Vaseeharan, Baskaralingam ;
Abinaya, Muthukumar ;
Vijayakumar, Sekar ;
Govindarajan, Marimuthu ;
Alharbi, Naiyf S. ;
Kadaikunnan, Shine ;
Khaled, Jamal M. ;
Benelli, Giovanni .
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2018, 178 :211-218
[2]   Treatment of candidemia and invasive candidiasis in the intensive care unit: post hoc analysis of a randomized, controlled trial comparing micafungin and liposomal amphotericin B [J].
Dupont, Bertrand F. ;
Lortholary, Olivier ;
Ostrosky-Zeichner, Luis ;
Stucker, Flavie ;
Yeldandi, Vijay .
CRITICAL CARE, 2009, 13 (05)
[3]  
Fekete-Forgács K, 1999, J BASIC MICROB, V39, P305, DOI 10.1002/(SICI)1521-4028(199912)39:5/6<305::AID-JOBM305>3.0.CO
[4]  
2-#
[5]   Attributable mortality of nosocomial candidemia, revisited [J].
Gudlaugsson, O ;
Gillespie, S ;
Lee, K ;
Berg, JV ;
Hu, JF ;
Messer, S ;
Herwaldt, L ;
Pfaller, M ;
Diekema, D .
CLINICAL INFECTIOUS DISEASES, 2003, 37 (09) :1172-1177
[6]   Management of invasive candidiasis and candidemia in adult non-neutropenic intensive care unit patients: Part I. Epidemiology and diagnosis [J].
Guery, Benoit P. ;
Arendrup, Maiken C. ;
Auzinger, Georg ;
Azoulay, Elie ;
Borges Sa, Marcio ;
Johnson, Elizabeth M. ;
Mueller, Eckhard ;
Putensen, Christian ;
Rotstein, Coleman ;
Sganga, Gabriele ;
Venditti, Mario ;
Zaragoza Crespo, Rafael ;
Kullberg, Bart Jan .
INTENSIVE CARE MEDICINE, 2009, 35 (01) :55-62
[7]   Transmission electron microscopy in molecular structural biology: A historical survey [J].
Harris, J. Robin .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2015, 581 :3-18
[8]   Comparison of Microdilution and Disk Diffusion Methods for the Detection of Fluconazole and Voriconazole Susceptibility Against Clinical Candida glabrata Isolates and Determination of Changing Susceptibility with New CLSI Breakpoints [J].
Hazirolan, Gulsen ;
Saribas, Zeynep ;
Arikan Akdagli, Sevtap .
MIKROBIYOLOJI BULTENI, 2016, 50 (03) :428-437
[9]   European expert opinion on the management of invasive candidiasis in adults [J].
Kullberg, B. J. ;
Verweij, P. E. ;
Akova, M. ;
Arendrup, M. C. ;
Bille, J. ;
Calandra, T. ;
Cuenca-Estrella, M. ;
Herbrecht, R. ;
Jacobs, F. ;
Kalin, M. ;
Kibbler, C. C. ;
Lortholary, O. ;
Martino, P. ;
Meis, J. F. ;
Munoz, P. ;
Odds, F. C. ;
De Pauw, B. E. ;
Rex, J. H. ;
Roilides, E. ;
Rogers, T. R. ;
Ruhnke, M. ;
Ullmann, A. J. ;
Uzun, O. ;
Vandewoude, K. ;
Vincent, J. -L. ;
Donnelly, J. P. .
CLINICAL MICROBIOLOGY AND INFECTION, 2011, 17 :1-12
[10]   Oral Azole Antifungal Medications and Risk of Acute Liver Injury, Overall and by Chronic Liver Disease Status [J].
Lo Re, Vincent, III ;
Carbonari, Dena M. ;
Lewis, James D. ;
Forde, Kimberly A. ;
Goldberg, David S. ;
Reddy, K. Rajender ;
Haynes, Kevin ;
Roy, Jason A. ;
Sha, Daohang ;
Marks, Amy R. ;
Schneider, Jennifer L. ;
Strom, Brian L. ;
Corley, Douglas A. .
AMERICAN JOURNAL OF MEDICINE, 2016, 129 (03) :283-+