Cytotoxic Effects of Transthyretin Aggregates in an Epidermoid Cell Line

被引：6

作者：

Fong, Vai Hong ^{[1
]}

Vieira, Amandio ^{[2
]}

机构：

[1] Eastern Mem Hosp, Dept Neurol, New Taipei, Taiwan

[2] Simon Fraser Univ, Dept Biomed Physiol BPK 9625, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada

来源：

PATHOBIOLOGY | 2017年 / 84卷 / 04期

关键词：

Amyloidogenic diseases; Catalase; Glutathione; Oxidation; Transthyretin; Reactive oxygen species; Epidermoid cells; FAMILIAL AMYLOID POLYNEUROPATHY; CARPAL-TUNNEL-SYNDROME; OXIDATIVE STRESS; BETA; GLUTATHIONE; INHIBITION; GENERATION; PEPTIDES; RECEPTOR; BRAIN;

D O I：

10.1159/000466701

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Objective: Skin amyloid deposits can occur as part of systemic amyloidoses including those involving misfolded-aggregated transthyretins (agTTR). Pathological effects of agTTR on the skin are not well understood. The main objective of the current study was to examine the toxicity of agTTR upon a human keratinocyte cell line. Methods: Cells were analyzed for indicators of oxidative stress after treatment with normal soluble TTR or the same pre-aggregation concentration of agTTR. Hydrogen peroxide production was analyzed as an indicator for the involvement of reactive oxygen species. Results: Treatment of cells with agTTR significantly increased hydrogen peroxide production (p < 0.05 vs. controls). Glutathione (GSH) and catalase were analyzed as indicators of endogenous cellular antioxidant activity. Treatment of cells with agTTR resulted in significant decreases in both catalase activity and GSH levels (p < 0.05 vs. controls). Conclusion: agTTR disrupts redox balance and induces oxidative stress in these epidermoid cells. (C) 2017 S. Karger AG, Basel

引用

页码：218 / 222

页数：5

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