Isolation of genomic DNA from medicinal plants without liquid nitrogen

被引：0
|
作者：
Sharma, Pratibha [1 ]
Joshi, Neha [1 ]
Sharma, Anubhuti [1 ]
机构：
[1] Banasthali Univ, Dept Biosci & Biotechnol, Banasthali 304022, India
来源：
INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY | 2010年 / 48卷 / 06期
关键词：
CTAB; DNA isolation; Liquid nitrogen; PCR; RAPD;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Genomic DNA was extracted from eight medicinal plants using the present DNA extraction protocols (CTAB extraction method) with some modifications. Leaves were fixed in different fixing solutions containing absolute alcohol (99.99%), chloroform and EDTA, but without liquid nitrogen. DNA quality and quantity obtained were comparable to those isolated with liquid nitrogen, as the lambda(260)/lambda(280) ratio with liquid nitrogen was in range 1.3-1.7 and with other fixing solutions it was 1.1-1.5. Absolute alcohol showed best results as fixing solution. Good quality of DNA was isolated without using liquid nitrogen from different medicinal plant species. DNA isolated by this method was suitable for various molecular biology applications.
引用
收藏
页码：610 / 614
页数：5
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