Type IX secretion system is pivotal for expression of gingipain-associated virulence of Porphyromonas gingivalis

被引:19
作者
Benedyk, Malgorzata [1 ,2 ]
Marczyk, Agata [1 ]
Chruscicka, Barbara [2 ]
机构
[1] Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Dept Microbiol, Krakow, Poland
[2] Jagiellonian Univ, Malopolska Ctr Biotechnol, Krakow, Poland
关键词
fitness; gingipain; periodontitis; Porphyromonas gingivalis; protein secretion; T9SS; virulence; POLYMICROBIAL SYNERGY; HOST RESPONSE; PROTEIN; MODEL; IDENTIFICATION; PERIODONTITIS; DYSBIOSIS; COMPLEX; BAD;
D O I
10.1111/omi.12268
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Porphyromonas gingivalis, a keystone pathogen in periodontitis, secretes an array of virulence factors including gingipains via the type IX secretion system (T9SS). Inactivation of any component of the T9SS leads to the accumulation of secreted proteins in unprocessed and, in the case of progingipains, inactive forms in the periplasm. To cast light on the paradox that active gingipains are essential for P. gingivalis fitness in vivo but a functional T9SS is not (Frontiers in Cellular and Infection Microbiology, 2017, 7:378), we have compared virulence of wild-type P. gingivalis W83 and the gingipain-null strain with isogenic mutants deficient in individual T9SS components. Using an in vivo subcutaneous chamber mouse model of infection, gingipain-null strain secretion mutants showed no virulence, but their pathogenic potential was reconstituted by coinfection with a low number of the parental strain. Apparently the same mechanism compensated fitness of mutants lacking functional T9SS the transposon library. In contrast to the parental strain, all mutants elicited significantly lower but an effective inflammatory immune response, which cleared infection and prevented systemic dissemination of P. gingivalis to organs. There were no significant differences in immune responses to different secretion mutants, which were generally more stimulatory than the gingipain-null strain. Together, these results indicate that functional T9SS is essential for P. gingivalis virulence apparently through delivery of active gingipains to the bacterial surface. Therefore, T9SS is a legitimate target for drug development to treat periodontitis.
引用
收藏
页码:237 / 244
页数:8
相关论文
共 50 条
  • [31] PG0026 Is the C-terminal Signal Peptidase of a Novel Secretion System of Porphyromonas gingivalis
    Glew, Michelle D.
    Veith, Paul D.
    Peng, Benjamin
    Chen, Yu-Yen
    Gorasia, Dhana G.
    Yang, Qiaohui
    Slakeski, Nada
    Chen, Dina
    Moore, Caroline
    Crawford, Simon
    Reynolds, Eric C.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (29) : 24605 - 24617
  • [32] Differential virulence and innate immune interactions of type I and II fimbrial genotypes of Porphyromonas gingivalis
    Wang, M.
    Liang, S.
    Hosur, K. B.
    Domon, H.
    Yoshimura, F.
    Amano, A.
    Hajishengallis, G.
    ORAL MICROBIOLOGY AND IMMUNOLOGY, 2009, 24 (06): : 478 - 484
  • [33] Riemerella anatipestifer GldG is necessary for secretion of effectors by type IX secretion system
    Chen, Zongchao
    Han, Wenlong
    Zhu, Min
    Liu, Dan
    Niu, Pengfei
    Shen, Ruyu
    Ding, Chan
    Yu, Shengqing
    VETERINARY MICROBIOLOGY, 2023, 276
  • [34] The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium psychrophilum
    Barbier, Paul
    Rochat, Tatiana
    Mohammed, Haitham H.
    Wiens, Gregory D.
    Bernardet, Jean-Francois
    Halpern, David
    Duchaud, Eric
    McBride, Mark J.
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2020, 86 (16) : 1 - 22
  • [35] The Type IX Secretion System: Advances in Structure, Function and Organisation
    Gorasia, Dhana G.
    Veith, Paul D.
    Reynolds, Eric C.
    MICROORGANISMS, 2020, 8 (08) : 1 - 9
  • [36] Type III secretion system genes of Edwardsiella tarda associated with intracellular replication and virulence in zebrafish
    Okuda, Jun
    Takeuchi, Yusuke
    Nakai, Toshihiro
    DISEASES OF AQUATIC ORGANISMS, 2014, 111 (01) : 31 - 39
  • [37] Susceptibility of type 2 diabetic mice to low-virulence bacterial infection:: induction of abscess formation by gingipain-deficient Porphyromonas gingivalis
    Ishihara, Y.
    Anan, H.
    Yoneda, M.
    Maeda, K.
    Hirofuji, T.
    JOURNAL OF PERIODONTAL RESEARCH, 2007, 42 (03) : 253 - 258
  • [38] Porphyromonas gingivalis virulence factors and invasion of cells of the cardiovascular system
    Progulske-Fox, A
    Kozarov, E
    Dorn, B
    Dunn, W
    Burks, J
    Wu, Y
    JOURNAL OF PERIODONTAL RESEARCH, 1999, 34 (07) : 393 - 399
  • [39] The outer-membrane export signal of Porphyromonas gingivalis type IX secretion system (T9SS) is a conserved C-terminal β-sandwich domain
    de Diego, Inaki
    Ksiazek, Miroslaw
    Mizgalska, Danuta
    Koneru, Lahari
    Golik, Przemyslaw
    Szmigielski, Borys
    Nowak, Magdalena
    Nowakowska, Zuzanna
    Potempa, Barbara
    Houston, John A.
    Enghild, Jan J.
    Thogersen, Ida B.
    Gao, Jinlong
    Kwan, Ann H.
    Trewhella, Jill
    Dubin, Grzegorz
    Xavier Gomis-Rueth, F.
    Ky-Anh Nguyen
    Potempa, Jan
    SCIENTIFIC REPORTS, 2016, 6
  • [40] Flavobacterium gliding motility and the type IX secretion system
    McBride, Mark J.
    Nakane, Daisuke
    CURRENT OPINION IN MICROBIOLOGY, 2015, 28 : 72 - 77