An antioxidant resveratrol significantly enhanced replication of hepatitis C virus

被引:57
作者
Nakamura, Mitsuyasu [1 ,2 ]
Saito, Hidetsugu [1 ]
Ikeda, Masanori [3 ]
Hokari, Ryota [2 ]
Kato, Nobuyuki [3 ]
Hibi, Toshifumi [1 ]
Miura, Soichiro [2 ]
机构
[1] Keio Univ, Dept Internal Med, Sch Med, Shinjuku Ku, Tokyo 1608582, Japan
[2] Natl Def Med Coll, Dept Internal Med 2, Tokorozawa, Saitama 3598513, Japan
[3] Okayama Univ, Dept Mol Biol, Grad Sch Med & Dent, Okayama 7008558, Japan
关键词
Replicon system; Luciferase assay; Ribavirin; Interferon; Polyphenol; Astaxanthin; OXIDATIVE STRESS; LIPID-METABOLISM; STELLATE CELLS; IN-VITRO; GENE-EXPRESSION; CLINICAL-TRIAL; MICE; INHIBITION; ASTAXANTHIN; INTERFERON;
D O I
10.3748/wjg.v16.i2.184
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To elucidate the effect of antioxidants, resveratrol (RVT) and astaxanthin (AXN), on hepatitis C virus (HCV) replication. METHODS: We investigated the effect of recent popular antioxidant supplements on replication of the HCV replicon system OR6. RVT is a strong antioxidant and a kind of polyphenol that inhibits replication of various viruses. AXN is also a strong antioxidant. The replication of HCV RNA was assessed by the luciferase reporter assay. An additive effect of antioxidants on antiviral effects of interferon (IFN) and ribavirin (RBV) was investigated. RESULTS: This is the first report to investigate the effect of RVT and AXN on HCV replication. In contrast to other reported viruses, RVT significantly enhanced HCV RNA replication. Vitamin E also enhanced HCV RNA replication as reported previously, although AXN did not affect replication. IFN and RBV significantly reduced HCV RNA replication, but these effects were dose-dependently hampered and attenuated by the addition of RVT. AXN did not affect antiviral effects of IFN or RBV, CONCLUSION: These results suggested that RVT is not suitable as an antioxidant therapy for chronic hepatitis C. (C) 2010 Baishideng. All rights reserved.
引用
收藏
页码:184 / 192
页数:9
相关论文
共 44 条
[1]   Dietary resveratrol alters lipid metabolism-related gene expression of mice on an atherogenic diet [J].
Ahn, Jiyun ;
Cho, Iljin ;
Kim, Suna ;
Kwon, Daeyoung ;
Ha, Taeyoul .
JOURNAL OF HEPATOLOGY, 2008, 49 (06) :1019-1028
[2]   Astaxanthin improves muscle lipid metabolism in exercise via inhibitory effect of oxidative CPT I modification [J].
Aoi, Wataru ;
Naito, Yuji ;
Takanami, Yoshikazu ;
Ishii, Takeshi ;
Kawai, Yukari ;
Akagiri, Satomi ;
Kato, Yoji ;
Osawa, Toshihiko ;
Yoshikawa, Toshikazu .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2008, 366 (04) :892-897
[3]  
Baroni GS, 1998, HEPATOLOGY, V27, P720
[4]   Astaxanthin and peridinin inhibit oxidative damage in Fe2+-loaded liposomes:: Scavenging oxyradicals or changing membrane permeability? [J].
Barros, MP ;
Pinto, E ;
Colepicolo, P ;
Pedersén, M .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 288 (01) :225-232
[5]   Resveratrol improves health and survival of mice on a high-calorie diet [J].
Baur, Joseph A. ;
Pearson, Kevin J. ;
Price, Nathan L. ;
Jamieson, Hamish A. ;
Lerin, Carles ;
Kalra, Avash ;
Prabhu, Vinayakumar V. ;
Allard, Joanne S. ;
Lopez-Lluch, Guillermo ;
Lewis, Kaitlyn ;
Pistell, Paul J. ;
Poosala, Suresh ;
Becker, Kevin G. ;
Boss, Olivier ;
Gwinn, Dana ;
Wang, Mingyi ;
Ramaswamy, Sharan ;
Fishbein, Kenneth W. ;
Spencer, Richard G. ;
Lakatta, Edward G. ;
Le Couteur, David ;
Shaw, Reuben J. ;
Navas, Placido ;
Puigserver, Pere ;
Ingram, Donald K. ;
de Cabo, Rafael ;
Sinclair, David A. .
NATURE, 2006, 444 (7117) :337-342
[6]   Resveratrol amplifies profibrogenic effects of free fatty acids on human hepatic stellate cells [J].
Bechmann, Lars P. ;
Zahn, Denis ;
Gieseler, Robert K. ;
Fingas, Christian D. ;
Marquitan, Guido ;
Jochum, Christoph ;
Gerken, Guido ;
Friedman, Scott L. ;
Canbay, Ali .
HEPATOLOGY RESEARCH, 2009, 39 (06) :601-608
[7]   How does SIRT1 affect metabolism, senescence and cancer? [J].
Brooks, Christopher L. ;
Gu, Wei .
NATURE REVIEWS CANCER, 2009, 9 (02) :123-128
[8]   Effect of resveratrol on alcohol-induced mortality and liver lesions in mice [J].
Bujanda, Luis ;
García-Barcina, María ;
Gutierrez-de Juan, Virginia ;
Bidaurrazaga, Joseba ;
Fernandez de Luco, Marian ;
Gutierrez-Stampa, Marian ;
Larzabal, Mikel ;
Hijona, Elisabeth ;
Sarasqueta, Cristina ;
Echenique-Elizondo, Miguel ;
Arenas, Juan I. .
BMC GASTROENTEROLOGY, 2006, 6 (1)
[9]   Complementary and alternative therapies in the treatment of chronic hepatitis C: a systematic review [J].
Coon, JT ;
Ernst, E .
JOURNAL OF HEPATOLOGY, 2004, 40 (03) :491-500
[10]   Statins Potentiate the In Vitro Anti-Hepatitis C Virus Activity of Selective Hepatitis C Virus Inhibitors and Delay or Prevent Resistance Development [J].
Delang, Leen ;
Paeshuyse, Jan ;
Vliegen, Inge ;
Leyssen, Pieter ;
Obeid, Susan ;
Durantel, David ;
Zoulim, Fabien ;
de Beeck, Anne Op ;
Neyts, Johan .
HEPATOLOGY, 2009, 50 (01) :6-16