Kinetic analysis of the reaction catalyzed by bacteriophage T7 RNA polymerase

被引：0

作者：

Pozhitkov, AE ^{[1
]}

Lavrik, IN

Sergeev, MM

Kochetkov, SN

机构：

[1] Moscow MV Lomonosov State Univ, Sch Chem, Moscow 119899, Russia

[2] Russian Acad Sci, Inst Mol Biol, Moscow 117984, Russia

来源：

MOLECULAR BIOLOGY | 1998年 / 32卷 / 01期

关键词：

bacteriophage T7 RNA polymerase; kinetic analysis; mathematical modeling;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The kinetics of the reaction catalyzed by bacteriophage T7 RNA polymerase was analyzed theoretically. A reaction scheme taking into account all major steps of the transcription cycle was proposed. By mathematical modeling, equations were derived describing the reaction rate as a function of the concentrations of initiating and noninitiating NTPs and promoter-containing template. Conditions were established in which the reaction catalyzed by T7 RNA polymerase obeys the Michaelis-Menten equation.

引用

页码：78 / 82

页数：5