Kinetic analysis of the reaction catalyzed by bacteriophage T7 RNA polymerase

被引：0
作者：
Pozhitkov, AE [1 ]
Lavrik, IN
Sergeev, MM
Kochetkov, SN
机构：
[1] Moscow MV Lomonosov State Univ, Sch Chem, Moscow 119899, Russia
[2] Russian Acad Sci, Inst Mol Biol, Moscow 117984, Russia
来源：
MOLECULAR BIOLOGY | 1998年 / 32卷 / 01期
关键词：
bacteriophage T7 RNA polymerase; kinetic analysis; mathematical modeling;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The kinetics of the reaction catalyzed by bacteriophage T7 RNA polymerase was analyzed theoretically. A reaction scheme taking into account all major steps of the transcription cycle was proposed. By mathematical modeling, equations were derived describing the reaction rate as a function of the concentrations of initiating and noninitiating NTPs and promoter-containing template. Conditions were established in which the reaction catalyzed by T7 RNA polymerase obeys the Michaelis-Menten equation.
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页码：78 / 82
页数：5
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