Circular RNA hsa_circ_0000511 Improves Epithelial Mesenchymal Transition of Cervical Cancer by Regulating hsa-mir-296-5p/HMGA1

被引:16
作者
Xie, Jia [1 ]
Chen, Qian [1 ]
Zhou, Ping [2 ]
Fan, Wenli [2 ]
机构
[1] Hosp Chengdu Univ Tradit Chinese Med, Dept Gynecol, Chengdu, Peoples R China
[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Childrens Hosp, Wuhan Maternal & Child Hlthcare Hosp, Wuhan, Peoples R China
关键词
E-CADHERIN PROMOTES; CELL CARCINOMA; GASTRIC-CANCER; PROLIFERATION; PROGRESSION; METASTASIS; EXPRESSION; MORTALITY; HMGA1;
D O I
10.1155/2021/9964538
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
As the second largest gynecological cancer, cervical cancer has been widely reported in recent years in which circular RNA is involved in the disease process. We earlier found that the expression of hsa_circ_0000511 in cervical cancer cells increased significantly, but its role in the process of cervical cancer is not clear. The purpose of this study is to explore its possible mechanisms in cervical cancer. Quantitative reverse transcription polymerase chain reaction (qRT-PCR), cell counting kit-8 assay, Transwell test, cell transfection, RNA pull-down assay and dual-luciferase reporter assay, and Western blot analysis were used to detect the expression and distribution of hsa_circ_0000511 in SiHa and HeLa cells, the ability of invasion and proliferation, and the modulated relationships between hsa_circ_0000511 and hsa-mir-296-5p, hsa-mir-296-5p, and HMGA1. hsa_circ_0000511 had the highest expression in SiHa and HeLa cells, and the expression in the cytoplasm was significantly higher than that in the nucleus, and its expression was not affected by RNase R. When hsa_circ_0000511 was silenced, its expression in SiHa and HeLa cells was significantly decreased; the proliferation, invasion, and migration abilities of the two kinds of cells were significantly enhanced; and the protein expression of E-cadherin was significantly upregulated, while the protein expression of N-cadherin was significantly downregulated. The expression of hsa-mir-296-5p was lower in SiHa and HeLa cells; however, its expression was increased when hsa_circ_0000511 was inhibited and decreased when hsa_circ_0000511 was overexpressed, so did the ability of proliferation, invasion, and migration and the protein expression of E-cadherin. Interestingly, the protein expression of HMGA1 also changed in these two cells when hsa-mir-296-5p was inhibited or overexpressed. Our results indicate that the upregulated hsa_circ_0000511 can inhibit the proliferation, invasion, and migration of SiHa and HeLa cells by regulating hsa-mir-296-5p/HMGA1, suggesting that the hsa_circ_0000511/hsa-mir-296-5p/HMGA1 pathway may be a potential target for the treatment of cervical cancer.
引用
收藏
页数:17
相关论文
共 50 条
  • [31] Circular RNA hsa_circ_0023404 exerts an oncogenic role in cervical cancer through regulating miR-136/TFCP2/YAP pathway
    Zhang, Jianhua
    Zhao, Xinyuan
    Zhang, Jin
    Zheng, Xuerong
    Li, Fenxia
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 501 (02) : 428 - 433
  • [32] Hsa_circ_0000515 is a novel circular RNA implicated in the development of breast cancer through its regulation of the microRNA-296-5p/CXCL10 axis
    Cai, Fenglin
    Fu, Wenjie
    Tang, Lei
    Tang, Jinhai
    Sun, Jinming
    Fu, Guangshun
    Ye, Gang
    FEBS JOURNAL, 2021, 288 (03) : 861 - 883
  • [33] Hsa_circ_0134111 promotes osteoarthritis progression by regulating miR-224-5p/CCL1 interaction
    Liu, Yongbao
    Zhang, Yanxiu
    AGING-US, 2021, 13 (16): : 20383 - 20394
  • [34] Hsa_circ_0000081 promotes the function of gastric cancer through sponging hsa-miR-423-5p to influence 3-phosphoinositide-dependent kinase 1 expression
    Jiang, Fei
    Hu, Xueju
    Cao, Hongyong
    Shen, Xiaobing
    BIOENGINEERED, 2022, 13 (04) : 8277 - 8290
  • [35] Circular RNA hsa_circ_ 0004650 Enhances 5-Fluorouracil Resistance in Gastric Cancer via Sponging miR-145-5p
    Zhou, Fusheng
    Zhuo, Jialong
    Xu, Xudong
    Pan, Duyi
    Cai, Chenwen
    Huang, Jiaxin
    Zhao, Xianguang
    Mao, Qiqi
    Jiang, Xiaoyun
    Sun, Xu
    Zhong, Liang
    Gao, Nan
    Chen, Jiajie
    ANTICANCER RESEARCH, 2025, 45 (01) : 173 - 187
  • [36] Circular RNA Circ-03955 Promotes Epithelial-Mesenchymal Transition in Osteosarcoma by Regulating miR-3662/Metadherin Pathway
    Wang, Zhengguang
    Deng, Mingsi
    Chen, Liangjian
    Wang, Weiguo
    Liu, Gengyan
    Liu, Dongbiao
    Han, Zhipeng
    Zhou, Yong
    FRONTIERS IN ONCOLOGY, 2020, 10
  • [37] A novel circular RNA hsa_circ_0008035 contributes to gastric cancer tumorigenesis through targeting the miR-375/YBX1 axis
    Huang, Shifang
    Zhang, Xiaolin
    Guan, Bingsheng
    Sun, Peng
    Hong, Chong Tsz
    Peng, Juzheng
    Tang, Shaohui
    Yang, Jingge
    AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH, 2019, 11 (04): : 2455 - 2462
  • [38] circ_0025033 promotes ovarian cancer development via regulating the hsa_miR-370-3p/SLC1A5 axis
    Ma, Huiping
    Qu, Shuyun
    Zhai, Yao
    Yang, Xiaofeng
    CELLULAR & MOLECULAR BIOLOGY LETTERS, 2022, 27 (01)
  • [39] hsa_circ_0087100/hsa-miR-6743-5p affects Th1 cell differentiation by regulating STAT1 in diabetic retinopathy
    He, Shuai
    Lai, Dongwei
    Ma, Chenggong
    Meng, Chunren
    Cai, Chunyang
    Chen, Qian
    Gu, Chufeng
    Qiu, Qinghua
    EPIGENOMICS, 2024, 16 (07) : 427 - 444
  • [40] RETRACTED ARTICLE: Circular RNA hsa_circ_0001829 promotes gastric cancer progression through miR-155-5p/SMAD2 axis
    Qiuling Niu
    Zhijie Dong
    Min Liang
    Yuanwei Luo
    Hai Lin
    Mingzhen Lin
    Xiu Zhong
    Wenxia Yao
    Jinsheng Weng
    Xinke Zhou
    Journal of Experimental & Clinical Cancer Research, 39