Circular RNA hsa_circ_0000511 Improves Epithelial Mesenchymal Transition of Cervical Cancer by Regulating hsa-mir-296-5p/HMGA1

被引:18
作者
Xie, Jia [1 ]
Chen, Qian [1 ]
Zhou, Ping [2 ]
Fan, Wenli [2 ]
机构
[1] Hosp Chengdu Univ Tradit Chinese Med, Dept Gynecol, Chengdu, Peoples R China
[2] Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Childrens Hosp, Wuhan Maternal & Child Hlthcare Hosp, Wuhan, Peoples R China
关键词
E-CADHERIN PROMOTES; CELL CARCINOMA; GASTRIC-CANCER; PROLIFERATION; PROGRESSION; METASTASIS; EXPRESSION; MORTALITY; HMGA1;
D O I
10.1155/2021/9964538
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
As the second largest gynecological cancer, cervical cancer has been widely reported in recent years in which circular RNA is involved in the disease process. We earlier found that the expression of hsa_circ_0000511 in cervical cancer cells increased significantly, but its role in the process of cervical cancer is not clear. The purpose of this study is to explore its possible mechanisms in cervical cancer. Quantitative reverse transcription polymerase chain reaction (qRT-PCR), cell counting kit-8 assay, Transwell test, cell transfection, RNA pull-down assay and dual-luciferase reporter assay, and Western blot analysis were used to detect the expression and distribution of hsa_circ_0000511 in SiHa and HeLa cells, the ability of invasion and proliferation, and the modulated relationships between hsa_circ_0000511 and hsa-mir-296-5p, hsa-mir-296-5p, and HMGA1. hsa_circ_0000511 had the highest expression in SiHa and HeLa cells, and the expression in the cytoplasm was significantly higher than that in the nucleus, and its expression was not affected by RNase R. When hsa_circ_0000511 was silenced, its expression in SiHa and HeLa cells was significantly decreased; the proliferation, invasion, and migration abilities of the two kinds of cells were significantly enhanced; and the protein expression of E-cadherin was significantly upregulated, while the protein expression of N-cadherin was significantly downregulated. The expression of hsa-mir-296-5p was lower in SiHa and HeLa cells; however, its expression was increased when hsa_circ_0000511 was inhibited and decreased when hsa_circ_0000511 was overexpressed, so did the ability of proliferation, invasion, and migration and the protein expression of E-cadherin. Interestingly, the protein expression of HMGA1 also changed in these two cells when hsa-mir-296-5p was inhibited or overexpressed. Our results indicate that the upregulated hsa_circ_0000511 can inhibit the proliferation, invasion, and migration of SiHa and HeLa cells by regulating hsa-mir-296-5p/HMGA1, suggesting that the hsa_circ_0000511/hsa-mir-296-5p/HMGA1 pathway may be a potential target for the treatment of cervical cancer.
引用
收藏
页数:17
相关论文
共 43 条
[1]   Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis [J].
Arbyn, Marc ;
Weiderpass, Elisabete ;
Bruni, Laia ;
de Sanjose, Silvia ;
Saraiya, Mona ;
Ferlay, Jacques ;
Bray, Freddie .
LANCET GLOBAL HEALTH, 2020, 8 (02) :E191-E203
[2]   Circular RNA hsa_circ_0000263 participates in cervical cancer development by regulating target gene of miR-150-5p [J].
Cai, Hongning ;
Zhang, Peng ;
Xu, Meng ;
Yan, Lin ;
Liu, Nian ;
Wu, Xufeng .
JOURNAL OF CELLULAR PHYSIOLOGY, 2019, 234 (07) :11391-11400
[3]   Rpph1 Upregulates CDC42 Expression and Promotes Hippocampal Neuron Dendritic Spine Formation by Competing with miR-330-5p [J].
Cai, Yifei ;
Sun, Ziling ;
Jia, Huizhen ;
Luo, Hongxue ;
Ye, Xiaoyang ;
Wu, Qi ;
Xiong, Yi ;
Zhang, Wei ;
Wan, Jun .
FRONTIERS IN MOLECULAR NEUROSCIENCE, 2017, 10
[4]   Mortality impact of achieving WHO cervical cancer elimination targets: a comparative modelling analysis in 78 low-income and lower-middle-income countries [J].
Canfell, Karen ;
Kim, Jane J. ;
Brisson, Marc ;
Keane, Adam ;
Simms, Kate T. ;
Caruana, Michael ;
Burger, Emily A. ;
Martin, Dave ;
Nguyen, Diep T. N. ;
Benard, Elodie ;
Sy, Stephen ;
Regan, Catherine ;
Drolet, Melanie ;
Gingras, Guillaume ;
Laprise, Jean-Francois ;
Torode, Julie ;
Smith, Megan A. ;
Fidarova, Elena ;
Trapani, Dario ;
Bray, Freddie ;
Ilbawi, Andre ;
Broutet, Nathalie ;
Hutubessy, Raymond .
LANCET, 2020, 395 (10224) :591-603
[5]   miR-214 activates TP53 but suppresses the expression of RELA, CTNNB1, and STAT3 in human cervical and colorectal cancer cells [J].
Chandrasekaran, Karthik Subramanian ;
Sathyanarayanan, Anusha ;
Karunagaran, Devarajan .
CELL BIOCHEMISTRY AND FUNCTION, 2017, 35 (07) :464-471
[6]   The biogenesis and emerging roles of circular RNAs [J].
Chen, Ling-Ling .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2016, 17 (04) :205-211
[7]   NCLscan: accurate identification of non-co-linear transcripts (fusion, trans-splicing and circular RNA) with a good balance between sensitivity and precision [J].
Chuang, Trees-Juen ;
Wu, Chan-Shuo ;
Chen, Chia-Ying ;
Hung, Li-Yuan ;
Chiang, Tai-Wei ;
Yang, Min-Yu .
NUCLEIC ACIDS RESEARCH, 2016, 44 (03)
[8]   HPV vaccines can be the hallmark of cancer prevention [J].
de Sanjose, Silvia ;
Delany-Moretlwe, Sinead .
LANCET, 2019, 394 (10197) :450-451
[9]  
Dong HY, 2020, AM J TRANSL RES, V12, P2781
[10]   Induction of tumor apoptosis through a circular RNA enhancing Foxo3 activity [J].
Du, William W. ;
Fang, Ling ;
Yang, Weining ;
Wu, Nan ;
Awan, Faryal Mehwish ;
Yang, Zhenguo ;
Yang, Burton B. .
CELL DEATH AND DIFFERENTIATION, 2017, 24 (02) :357-370