Theoretical explanation for the DNA cleavage by GO with cation: anti-cooperativity effect among the πMIDLINE HORIZONTAL ELLIPSISπ, cationMIDLINE HORIZONTAL ELLIPSISπ/σ and H-bonding interactions in cytosineMIDLINE HORIZONTAL ELLIPSISGOMIDLINE HORIZONTAL ELLIPSISMn+ (Mn+ = Na+, Mg2+, Al3+)*

In order to reveal the nature of DNA cleavage by inorganic intercalator GO (graphene oxide) with cation, the cooperativity effects among the pi MIDLINE HORIZONTAL ELLIPSIS pi, cationMIDLINE HORIZONTAL ELLIPSIS pi/sigma and H-bonding interactions were evaluated in the cytosineMIDLINE HORIZONTAL ELLIPSISGOMIDLINE HORIZONTAL ELLIPSISMn+ (Mn+ = Na+, Mg2+, Al3+) model systems using the M06-2X, MP2 and omega B97X-D methods with the 6-311++G(2d,p) and 6-311++G(3df,3pd) basis sets. The Mn+MIDLINE HORIZONTAL ELLIPSISO (ether) and N-HMIDLINE HORIZONTAL ELLIPSISO interactions induce the formation of the pi MIDLINE HORIZONTAL ELLIPSIS pi stacking between cytosine and GO, and the anti-cooperativity effect are dominant in controling of the aggregation process of cytosine, GO and Mn+, which was confirmed by the AIM (atoms-in-molecules) and RDG (reduced density gradient) analyses. Furthermore, the solvent effects of H2O weaken greatly the anti-cooperativity effects. Thus, a deduction on the DNA cleavage by GOMIDLINE HORIZONTAL ELLIPSIScation with the intercalation mode is put forward: due to the anti-cooperativity effect and solvent effect, the pi MIDLINE HORIZONTAL ELLIPSIS pi stacking is weakened in the complexes with Na+ or broken in those with Mg2+ or Al3+. Then the GOMIDLINE HORIZONTAL ELLIPSISMg2+ moiety is squeezed out from the intercalating sites, leading to an invalid cleavage of DNA, while Na+ or Al3+ is bound tightly to cytosine, with a notable DNA cleavage. This deduction was used to explain reasonably the previous experimental phenomena.