In vivo measurement of afferent activity with axon-specific calcium imaging

被引:132
作者
Broussard, Gerard Joey [1 ]
Liang, Yajie [2 ]
Fridman, Marina [3 ]
Unger, Elizabeth K. [1 ]
Meng, Guanghan [2 ,4 ]
Xiao, Xian [1 ,5 ]
Ji, Na [2 ,4 ]
Petreanu, Leopoldo [3 ]
Tian, Lin [1 ]
机构
[1] Univ Calif Davis, Dept Biochem & Mol Med, Davis, CA 95616 USA
[2] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA USA
[3] Champalimaud Ctr Unknown, Lisbon, Portugal
[4] Univ Calif Berkeley, Dept Phys, Dept Mol & Cellular Biol, Berkeley, CA 94720 USA
[5] Westlake Inst Adv Study, Hangzhou, Zhejiang, Peoples R China
基金
美国国家卫生研究院;
关键词
PRIMARY VISUAL-CORTEX; FLUORESCENT PROTEINS; CA2+ INDICATORS; NEURAL ACTIVITY; ACTION-POTENTIALS; NEURONAL-ACTIVITY; PLASMA-MEMBRANE; CELLS; DYNAMICS; SIGNAL;
D O I
10.1038/s41593-018-0211-4
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In vivo calcium imaging from axons provides direct interrogation of afferent neural activity, informing the neural representations that a local circuit receives. Unlike in somata and dendrites, axonal recording of neural activity-both electrically and optically-has been difficult to achieve, thus preventing comprehensive understanding of neuronal circuit function. Here we developed an active transportation strategy to enrich GCaMP6, a genetically encoded calcium indicator, uniformly in axons with sufficient brightness, signal-to-noise ratio, and photostability to allow robust, structure-specific imaging of presynaptic activity in awake mice. Axon-targeted GCaMP6 enables frame-to-frame correlation for motion correction in axons and permits subcellular-resolution recording of axonal activity in previously inaccessible deep-brain areas. We used axon-targeted GCaMP6 to record layer-specific local afferents without contamination from somata or from intermingled dendrites in the cortex. We expect that axon-targeted GCaMP6 will facilitate new applications in investigating afferent signals relayed by genetically defined neuronal populations within and across specific brain regions.
引用
收藏
页码:1272 / +
页数:12
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