Cloning of an orange-spotted grouper Epinephelus coioides heat shock protein 90AB (HSP90AB) and characterization of its expression in response to nodavirus

被引：40

作者：

Chen, Young-Mao ^{[1
,2
,3
]}

Kuo, Cham-En ^{[4
]}

Wang, Ting-Yu ^{[1
,2
,3
]}

Shie, Pei-Shiuan ^{[1
,2
,3
]}

Wang, Wei-Chen ^{[1
]}

Huang, Shao-Ling ^{[1
,2
,3
]}

Tsai, Tieh-Jung ^{[1
]}

Chen, Peng-Peng ^{[1
,2
,3
]}

Chen, Jiann-Chu ^{[5
]}

Chen, Tzong-Yueh ^{[1
,2
,3
]}

机构：

[1] Natl Cheng Kung Univ, Genet Mol Lab, Inst Biotechnol, Coll Biosci & Biotechnol, Tainan 70101, Taiwan

[2] Natl Cheng Kung Univ, Res Ctr Ocean Environm & Technol, Tainan 70101, Taiwan

[3] Natl Cheng Kung Univ, Agr Biotechnol Res Ctr, Tainan 70101, Taiwan

[4] Tzu Hui Inst Technol, Dept Nursing, Pingtung 926, Taiwan

[5] Natl Taiwan Ocean Univ, Dept Aquaculture, Chilung 202, Taiwan

来源：

FISH & SHELLFISH IMMUNOLOGY | 2010年 / 28卷 / 5-6期

关键词：

HSP90AB; Nodavirus; Grouper; Geldanamycin; OXIDATIVE STRESS; GENE-EXPRESSION; RNA; CHAPERONE; CDNA; CELLS; REPLICATION; ACTIVATION; INDUCTION; INFECTION;

D O I：

10.1016/j.fsi.2010.02.004

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The heat shock proteins (HSPs) family which consists of HSP90, HSP70, and low molecular mass HSPs are involved in chaperone activity. Here, we report the cloning and characterization of HSP90AB gene from orange-spotted grouper, Epinephelus coioides. The full-length of grouper HSP90AB was 727 amino acids and possessed an ATPase domain as well as an evolutionarily conserved molecular chaperone. The HSP90AB-green fluorescent protein fusion protein was evenly distributed in the cytoplasm. Immunohistochemistry (IHC) and real-time polymerase chain reaction (PCR) analyses indicated that the expression of grouper HSP90AB was marginally increased following nodavirus infection. Grouper E. coioides that received HSP90 inhibitor geldanamycin (GA) showed an increase in HSP90AB expression and growth of nodavirus supporting nodavirus replication. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：895 / 904

页数：10

共 46 条

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