Aldolase A Enhances Intrahepatic Cholangiocarcinoma Proliferation and Invasion through Promoting Glycolysis

被引：26
作者：
Li, Xiang [1 ,2 ]
Yu, Chang [2 ]
Luo, Yichun [2 ]
Lin, Jiacheng [2 ]
Wang, Fang [2 ]
Sun, Xuehua [2 ]
Gao, Yueqiu [2 ]
Tan, Weifeng [1 ]
Xia, Qiang [1 ]
Kong, Xiaoni [2 ]
机构：
[1] Shanghai Jiao Tong Univ, Renji Hosp, Sch Med, Dept Hepat Surg, Shanghai, Peoples R China
[2] Shanghai Univ Chinese Tradit Med, ShuGuang Hosp, Dept Liver Dis, Cent Lab, 528 Zhangheng Rd, Shanghai 201203, Peoples R China
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES | 2021年 / 17卷 / 07期
基金：
中国国家自然科学基金;
关键词：
Intrahepatic Cholangiocarcinoma; Aldolase A; Metabolism Reprogram; Enzymatic Activity; Glycolysis; PYRUVATE-KINASE M2; GLUCOSE-METABOLISM; CANCER METABOLISM; LUNG-CANCER; CELL; ITACONATE; GROWTH; HIF-1-ALPHA; SUPPRESSES; EGFR;
D O I：
10.7150/ijbs.59068
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Energy metabolism reprogramming has been implicated in tumorigenesis and development. Key metabolism enzyme Aldolase A (ALDOA) has been shown to be highly expressed and involved in various kinds of cancers including hepatocellular carcinoma. In this study, we found that ALDOA was highly expressed in clinical intrahepatic cholangiocarcinoma (ICC) tissues, and its high expression was negatively correlated with overall survival (OS) and recurrence-free survival (RFS) in ICC patients. Knockdown of ALDOA expression significantly inhibited the proliferation and migration of ICC both in vitro and in vivo, while highly-expressed ALDOA in ICC cells promoted the proliferation and migration of ICC cells. By applying ALDOA inhibitor and metabolic mass spectrometry tests, we demonstrated that ALDOA modulated the biological characteristics and metabolic level of ICC cells depending on its enzymatic activity. In summary, ALDOA promotes ICC proliferation and migration by enhancing ICC cells glycolysis. Blocking enzymatic activity of ALDOA provides a strategy to inhibit ICC.
引用
收藏
页码：1782 / 1794
页数：13
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