Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor

被引:23
作者
Chavez, Miquella G. [1 ,2 ,3 ]
Hu, Jimmy [1 ,2 ]
Seidel, Kerstin [1 ,2 ]
Li, Chunying [1 ,2 ,4 ,5 ]
Jheon, Andrew [1 ,2 ]
Naveau, Adrien [6 ,7 ,8 ]
Horst, Orapin [1 ,2 ,9 ]
Klein, Ophir D. [1 ,2 ,10 ,11 ]
机构
[1] Univ Calif San Francisco, Dept Orofacial Sci, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Program Craniofacial & Mesenchymal Biol, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94143 USA
[4] Dalian Univ, Dept Pathol, Dalian 116012, Peoples R China
[5] Dalian Univ, Zhongshan Hosp, Res Ctr, Dalian 116012, Peoples R China
[6] Univ Paris 05, Sorbonne Paris Cite, UMR S872, Paris, France
[7] Univ Paris 06, Ctr Rech Cordeliers, UMR S872, F-75252 Paris 05, France
[8] INSERM, U872, F-75654 Paris 13, France
[9] Univ Calif San Francisco, Dept Prevent & Restorat Dent Sci, Div Endodont, San Francisco, CA 94143 USA
[10] Univ Calif San Francisco, Dept Pediat, San Francisco, CA 94143 USA
[11] Univ Calif San Francisco, Inst Human Genet, San Francisco, CA 94143 USA
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2014年 / 87期
基金
美国国家卫生研究院;
关键词
Stem Cell Biology; Issue; 87; Epithelial Stem Cells; Adult Stem Cells; Incisor; Cervical Loop; Cell Culture; HUMAN EPIDERMAL-KERATINOCYTES; SELF-RENEWAL; ENAMEL ORGAN; RAT INCISOR; CULTIVATION; TEETH; SKIN; DIFFERENTIATION; MULTIPLICATION; PROGENITORS;
D O I
10.3791/51266
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Understanding the cellular and molecular mechanisms that underlie tooth regeneration and renewal has become a topic of great interest1-4, and the mouse incisor provides a model for these processes. This remarkable organ grows continuously throughout the animal's life and generates all the necessary cell types from active pools of adult stem cells housed in the labial (toward the lip) and lingual (toward the tongue) cervical loop (CL) regions. Only the dental stem cells from the labial CL give rise to ameloblasts that generate enamel, the outer covering of teeth, on the labial surface. This asymmetric enamel formation allows abrasion at the incisor tip, and progenitors and stem cells in the proximal incisor ensure that the dental tissues are constantly replenished. The ability to isolate and grow these progenitor or stem cells in vitro allows their expansion and opens doors to numerous experiments not achievable in vivo, such as high throughput testing of potential stem cell regulatory factors. Here, we describe and demonstrate a reliable and consistent method to culture cells from the labial CL of the mouse incisor.
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页数:7
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