Molecular adaptations of testosterone-producing Leydig cells during systemic in vivo blockade of the androgen receptor

被引：13

作者：

Bjelic, Maja M. ^{[1
]}

Stojkov, Natasa J. ^{[1
]}

Baburski, Aleksandar Z. ^{[1
]}

Sokanovic, Srdjan J. ^{[1
]}

Mihajlovic, Aleksandar I. ^{[1
]}

Janjic, Marija M. ^{[1
]}

Kostic, Tatjana S. ^{[1
]}

Andric, Silvana A. ^{[1
]}

机构：

[1] Univ Novi Sad, Fac Sci, Dept Biol & Ecol, Reprod Endocrinol & Signaling Grp, Novi Sad 21000, Serbia

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 2014年 / 396卷 / 1-2期

关键词：

Leydig cells; Testosterone; Androcur; Steroidogenic machinery; cAMP signaling; REGULATORY PROTEIN EXPRESSION; TESTICULAR INTERSTITIAL FLUID; RNA HELICASE GRTH/DDX25; STEROIDOGENIC MACHINERY; MITOCHONDRIAL PROTEIN; MEDIATED REGULATION; ANABOLIC THERAPIES; NITRIC-OXIDE; TUMOR-CELLS; HORMONE;

D O I：

10.1016/j.mce.2014.08.007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

This study systematically evaluates the effects of androgen receptor (AR) blockade on molecular events in Leydig cells. Results showed that intramuscular administration of testosterone-enanthate, at clinically relevant dose, decreased testosterone in interstitial fluid and Leydig cells from adult rats. AR-blocker (Androcur) prevented this effect and testosterone-reduced Leydig cells steroidogenic capacity/activity. Testosterone-reduced expression of some steroidogenic enzymes/proteins (Tspo,StAR,Hsd3b1/2) and transcription factors (Nur77,Gata4,Dax1) was completely abrogated, while decreased expression of Star,Cyp11a1,Cyp17a1,Hsd17b4,Creb1a was partially prevented. In the same cells, increased expression of Hsd3b5/HSD3B and Ar/AR was abolished. Androcur-treatment abolished testosterone-reduced cAMP, coupled with a changed expressional milieu of cAMP signaling elements. Results from in vitro experiments suggest that some of these effects are testosterone-AR dependent, while others could be due to disturbed LH and/or other signals. Presented data provide new molecular insight into Leydig cells function and are important in terms of human reproductive health and the wide-spread use of Androcur as well as use/abuse of testosterone-enanthate. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

引用

页码：10 / 25

页数：16

共 78 条

[1] Energized, polarized, and actively respiring mitochondria are required for acute Leydig cell steroidogenesis [J].