Silenced ABCE1 gene inhibited human gallbladder carcinoma GBC-SD cell proliferation, invasion and migration by electroporation method

To investigate the effect of silenced ABCE1 gene expression in human gallbladder carcinoma GBC-SD cell on proliferation, invasion and migration by electroporation method. Targeted ABCE1 siRNA sequenced (ABCE1-siRNA) as well as the negative control sequence (NC-si RNA) was designed and synthesized. They were transfected into human gallbladder carcinoma GBC-SD cells by electroporation method and the ABCE1-GBC-SD, NC-siRNA-GBC-SD cells were formed. By RT-PCR, Western blotting method, expression level of ABCE1 mRNA and protein in transfected cells were detected. Flow cytometry was used to detect GBC-SD cell cycle and apoptosis. CCK-8 proliferation assay, scratch healing assay, cell invasion assay were used to measured cell proliferation, migration, and invasion abilities. The expression level of ABCE1 m RNA and protein in ABCE1-GBC-SD cell group, compared with that in NC-siRNA-GBC-SD and empty vector cells group (Ctrl-GBC-SD cell group), were significantly decreased. Growth rate of the cells slowed down in ABCE1-GBC-SD group (P<0.05). The cell cycle was arrested in G0/G1 phase, and the number of cells in S phase were reduced; compared with empty vector group, apoptosis rate was significantly higher (P<0.01) in ABCE1-GBC-SD group and proliferation, migration as well as invasion were significantly decreased (P<0.05). The electroporation method silenced ABCE1 gene expression may inhibit proliferation, invasion and migration ability of gallbladder carcinoma GBC-SD cell.