Tetracycline Resistance Genes Identified from Distinct Soil Environments in China by Functional Metagenomics

被引:38
作者
Wang, Shaochen [1 ]
Gao, Xia [1 ]
Gao, Yuejiao [1 ]
Li, Yanqing [1 ]
Cao, Mingming [1 ]
Xi, Zhenhua [2 ]
Zhao, Lixing [3 ]
Feng, Zhiyang [1 ]
机构
[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing, Jiangsu, Peoples R China
[2] Chinese Acad Sci, Inst Tibetan Plateau Res, Beijing, Peoples R China
[3] Yunnan Univ, Yunnan Inst Microbiol, Kunming, Yunnan, Peoples R China
来源
FRONTIERS IN MICROBIOLOGY | 2017年 / 8卷
基金
中国国家自然科学基金;
关键词
functional metagenomics; soil; tetracycline resistance; efflux transporter; tetracycline destructase; ANTIBIOTIC-RESISTANCE; RESISTOME; DETERMINANT; BACTERIA; REVEALS; POULTRY; FOOD;
D O I
10.3389/fmicb.2017.01406
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Soil microbiota represents one of the ancient evolutionary origins of antibiotic resistance and has been increasingly recognized as a potentially vast unstudied reservoir of resistance genes with possibilities to exchange with pathogens. Tetracycline resistance is one of the most abundant antibiotic resistances that may transfer among clinical and commensal microorganisms. To investigate tetracycline resistance genes from soil bacteria in different habitats, we performed functional analysis of three metagenomic libraries derived from soil samples collected from Yunnan, Sichuan, and Tibet, respectively, in China. We found efflux transporter genes form all the libraries, including 21 major facilitator superfamily efflux pump genes and one multidrug and toxic compound extrusion (MATE) transporter gene. Interestingly, we also identified two tetracycline destructase genes, belonging to a newly described family of tetracycline-inactivating enzymes that scarcely observed in clinical pathogens, from the Tibet library. The inactivation activity of the putative enzyme was confirmed in vitro by biochemical analysis. Our results indicated that efflux pumps distributed predominantly across habitats. Meanwhile, the mechanism of enzymatic inactivation for tetracycline resistance should not be neglected and merits further investigation.
引用
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页数:9
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