Interaction of bovine serum albumin with poly(N-vinylazoles)

The interaction of bovine serum albumin with poly(1-vinylimidazole) and poly(l-vinyl-l,2,4-triazole) in an aqueous solution at varying pH values and ionic strengths was studied by potentiometric titration, turbidimetry, viscometry, IR spectroscopy, and microscopy. It was discovered that the interpolymer complexes stabilized by hydrogen bonds are formed in the systems under examination. In the system involving poly(1-vinylimidazole), a reversible phase separation, accompanied by precipitation of the complex, was shown to take place at pH 4.5-6. This effect may be used in the concentration and separation of complex albumin mixtures.